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Literature summary extracted from

  • Tokuyasu, K.; Ohnishi-Kameyama, M.; Hayashi, K.
    Purification and characterization of extracellular chitin deacetylase from Colletotrichum lindemuthianum (1996), Biosci. Biotechnol. Biochem., 60, 1598-1603.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.41 EDTA
-
 Colletotrichum lindemuthianum

Application

EC Number Application Comment Organism
3.5.1.41 synthesis industrial production of chitosan Colletotrichum lindemuthianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 Co2+ at 10 mM Colletotrichum lindemuthianum
3.5.1.41 Cu2+
-
 Colletotrichum lindemuthianum
3.5.1.41 Fe2+
-
 Colletotrichum lindemuthianum
3.5.1.41 Mn2+
-
 Colletotrichum lindemuthianum
3.5.1.41 Ni2+
-
 Colletotrichum lindemuthianum
3.5.1.41 Zn2+
-
 Colletotrichum lindemuthianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 0.414
-
 (N-acetyl-D-glucosamine)5
-
 Colletotrichum lindemuthianum
3.5.1.41 0.6
-
 (N-acetyl-D-glucosamine)4
-
 Colletotrichum lindemuthianum
3.5.1.41 2.55
-
 glycol chitin
-
 Colletotrichum lindemuthianum
3.5.1.41 11
-
 (N-acetyl-D-glucosamine)3
-
 Colletotrichum lindemuthianum
3.5.1.41 18.4
-
 (N-acetyl-D-glucosamine)2
-
 Colletotrichum lindemuthianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Colletotrichum lindemuthianum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Co2+ at 1 mM Colletotrichum lindemuthianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.41 31500
-
 SDS-PAGE Colletotrichum lindemuthianum
3.5.1.41 33000
-
 gel filtration Colletotrichum lindemuthianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.41 chitin + H2O Colletotrichum lindemuthianum
-
 chitosan + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Colletotrichum lindemuthianum
-
 ATCC 56676
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.41 glycoprotein
-
 Colletotrichum lindemuthianum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 944fold to homogeneity Colletotrichum lindemuthianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 culture filtrate
-
 Colletotrichum lindemuthianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 18.4
-
-
 Colletotrichum lindemuthianum

Storage Stability

EC Number Storage Stability Organism
3.5.1.41 4°C Colletotrichum lindemuthianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 (N-acetyl-D-glucosamine)2 + H2O
-
 Colletotrichum lindemuthianum (GlcN)GlcNAc + acetate
-
 ?
3.5.1.41 (N-acetyl-D-glucosamine)3
-
 Colletotrichum lindemuthianum (GlcN)2GlcNAc + acetate
-
 ?
3.5.1.41 (N-acetyl-D-glucosamine)4
-
 Colletotrichum lindemuthianum (GlcN)3GlcNAc + acetate
-
 ?
3.5.1.41 (N-acetyl-D-glucosamine)4 + H2O
-
 Colletotrichum lindemuthianum (GlcN)3GlcNAc + acetate
-
 ?
3.5.1.41 (N-acetyl-D-glucosamine)5
-
 Colletotrichum lindemuthianum (GlcN)4GlcNAc + acetate
-
 ?
3.5.1.41 (N-acetyl-D-glucosamine)5 + H2O
-
 Colletotrichum lindemuthianum (GlcN)4GlcNAc + acetate
-
 ?
3.5.1.41 chitin + H2O
-
 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 chitin + H2O partially N-deacetylated water soluble chitin Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 glycol chitin + H2O
-
 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 N,N',N'',N''',N''''-pentaacetylchitopentaose + 5 H2O
-
 Colletotrichum lindemuthianum chitopentaose + 5 acetate end product ?
3.5.1.41 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N',N''-triacetylchitotriose + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N'-diacetylchitobiose + H2O
-
 Colletotrichum lindemuthianum deacetylated chitooligosaccharides + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 monomer 1 * 31500-33000, SDS-PAGE Colletotrichum lindemuthianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 60
-
-
 Colletotrichum lindemuthianum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.41 additional information
-
-
 Colletotrichum lindemuthianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 additional information
-
-
 Colletotrichum lindemuthianum
3.5.1.41 45
-
 stable Colletotrichum lindemuthianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.41 5.94
-
 (N-acetyl-D-glucosamine)3
-
 Colletotrichum lindemuthianum
3.5.1.41 8.52
-
 (N-acetyl-D-glucosamine)2
-
 Colletotrichum lindemuthianum
3.5.1.41 27.2
-
 glycol chitin
-
 Colletotrichum lindemuthianum
3.5.1.41 83.8
-
 (N-acetyl-D-glucosamine)5
-
 Colletotrichum lindemuthianum
3.5.1.41 96.9
-
 (N-acetyl-D-glucosamine)4
-
 Colletotrichum lindemuthianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 11.5 12
-
 Colletotrichum lindemuthianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.41 additional information
-
-
 Colletotrichum lindemuthianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.41 additional information
-
-
 Colletotrichum lindemuthianum
3.5.1.41 5 10.5
-
 Colletotrichum lindemuthianum