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Literature summary extracted from

  • Tsigos, I.; Bouriotis, V.
    Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum (1995), J. Biol. Chem., 270, 26286-26291.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 Cu2+
-
 Colletotrichum lindemuthianum
3.5.1.41 Mn2+
-
 Colletotrichum lindemuthianum
3.5.1.41 Zn2+
-
 Colletotrichum lindemuthianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Colletotrichum lindemuthianum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Co2+ substrate: glycol chitosan Colletotrichum lindemuthianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.41 50000
-
 enzyme after deglycosylation, gel filtration, SDS-PAGE Colletotrichum lindemuthianum
3.5.1.41 150000
-
 amino acid and carbohydrate analysis in combination with data from SDS-PAGE and gel filtration Colletotrichum lindemuthianum
3.5.1.41 250000
-
 gel filtration, SDS-PAGE Colletotrichum lindemuthianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.41 chitin + H2O Colletotrichum lindemuthianum
-
 chitosan + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Colletotrichum lindemuthianum
-
 DSM 63144
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.41 glycoprotein galactose, mannose, xylose, fucose, N-acetyl-D-glucosamine, N-acetylgalactosamine, N-acetylneuraminic acid Colletotrichum lindemuthianum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 110fold to homogeneity Colletotrichum lindemuthianum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 culture filtrate
-
 Colletotrichum lindemuthianum
-
3.5.1.41 mycelium
-
 Colletotrichum lindemuthianum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 0.219
-
-
 Colletotrichum lindemuthianum

Storage Stability

EC Number Storage Stability Organism
3.5.1.41 -20°C Colletotrichum lindemuthianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 carboxymethylchitin + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 chitin + H2O
-
 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 chitin + H2O amorphous chitin Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 chitin + H2O chitin 50 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 glycol chitin + H2O
-
 Colletotrichum lindemuthianum chitosan + acetate
-
 ?
3.5.1.41 glycol chitosan + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N',N''-triacetylchitotriose + H2O high substrate concentration needed Colletotrichum lindemuthianum ?
-
 ?
3.5.1.41 N,N'-diacetylchitobiose + H2O high substrate concentration needed Colletotrichum lindemuthianum deacetylated chitooligosaccharides + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 monomer 1 * 150000, amino acid and carbohydrate analysis in combination with data from SDS-PAGE and gel filtration Colletotrichum lindemuthianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 50
-
-
 Colletotrichum lindemuthianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 additional information
-
-
 Colletotrichum lindemuthianum
3.5.1.41 50
-
 nearly stable for 45 h Colletotrichum lindemuthianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 8.5
-
-
 Colletotrichum lindemuthianum