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Literature summary extracted from

  • Oda, K.; Takahashi, T.; Tokuda, Y.; Shibano, Y.; Takahashi, S.
    Cloning, nucleotide sequence, and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from Pseudomonas sp. 101 (1994), J. Biol. Chem., 269, 26518-26524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.101 expression in Escherichia coli Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.101 0.0188
-
 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu recombinant PCP Pseudomonas sp.
3.4.21.101 0.0191
-
 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu autentic PCP Pseudomonas sp.
3.4.21.101 0.0823
-
 Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu recombinant PCP Pseudomonas sp.
3.4.21.101 0.0829
-
 Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu autentic PCP Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.101 61070
-
 calculation from PCP gene, processed and secreted into the periplasm as a 43000 protein, which, under acidic conditions converts to mature PCP Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.101 Pseudomonas sp.
-
-
-
3.4.21.101 Pseudomonas sp.
-
 expression in Escherichia coli
-
3.4.21.101 Pseudomonas sp. No. 101
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.101 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.101 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. No. 101 Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.101 Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.101 Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. No. 101 Lys-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.101 Pseudomonas carboxyl proteinase
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.101 19.2
-
 Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu autentic and recombinant PCP Pseudomonas sp.
3.4.21.101 20.4
-
 Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu autentic and recombinant PCP Pseudomonas sp.