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Literature summary extracted from

  • Oda, K.; Nakatani, H.; Dunn, B.M.
    Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101 (1992), Biochim. Biophys. Acta, 1120, 208-214.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.100 additional information not: pepstatin Pseudomonas sp.
3.4.21.100 Tyrostatin
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.100 additional information
-
 additional information overview: Km of synthetic substrates Pseudomonas sp.
3.4.21.100 0.003
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 0.013
-
 Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 0.019
-
 Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 0.052
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 0.06
-
 Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 0.095
-
 Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.100 Pseudomonas sp.
-
 No. 101
-
3.4.21.100 Pseudomonas sp. No. 101
-
 No. 101
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.100 Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Ala-Asp-Phe + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. No. 101 Lys-Pro-Ala-Asp-Phe + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Ala-Lys-Leu + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Ala-Lys-Leu-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. No. 101 Lys-Pro-Ala-Lys-Leu + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Ala-Lys-Phe-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Ala-Lys-Phe + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Asn-Gln-Phe-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Asn-Gln-Phe + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. No. 101 Lys-Pro-Ile-Glu-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.100 Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu + H2O
-
 Pseudomonas sp. Lys-Pro-Val-Ser-Tyr + 4-nitrophenylalanine-Arg-Leu
-
 ?
3.4.21.100 additional information substrate specificity against synthetic substrates: synthetic substrates with general structure P5-P4-P3-P2-P1*(4-nitro)Phe-Arg-Leu. The nature of the residue occupying the P2, P3 and P4 position as well as P1 position has strong influence on kinetic parameters Pseudomonas sp. ?
-
 ?
3.4.21.100 additional information substrate specificity against synthetic substrates: synthetic substrates with general structure P5-P4-P3-P2-P1*(4-nitro)Phe-Arg-Leu. The nature of the residue occupying the P2, P3 and P4 position as well as P1 position has strong influence on kinetic parameters Pseudomonas sp. No. 101 ?
-
 ?
3.4.21.100 Ser-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. Ser-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
-
 ?
3.4.21.100 Ser-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
-
 Pseudomonas sp. No. 101 Ser-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.100 additional information
-
 additional information overview: turnover number of synthetic substrates Pseudomonas sp.
3.4.21.100 0.0867
-
 Lys-Pro-Val-Ser-Tyr-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 5.5
-
 Lys-Pro-Asn-Gln-Phe-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 6.8
-
 Lys-Pro-Ala-Asp-Phe-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 6.9
-
 Lys-Pro-Ile-Glu-Phe-(4-nitro)Phe-Arg-Leu
-
 Pseudomonas sp.
3.4.21.100 14.7
-
 Lys-Pro-Ala-Lys-Phe-4-nitrophenylalanine-Arg-Leu
-
 Pseudomonas sp.