EC Number | General Stability | Organism |
---|---|---|
3.4.13.18 | stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.18 | 2-mercaptoethanol | - |
Escherichia coli | |
3.4.13.18 | Co2+ | when substrates are cleaved rapidly by dipeptidase, addition of Co2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect | Escherichia coli | |
3.4.13.18 | cysteine | - |
Escherichia coli | |
3.4.13.18 | D-Forms of dipeptides | - |
Escherichia coli | |
3.4.13.18 | DTT | - |
Escherichia coli | |
3.4.13.18 | iodoacetate | - |
Escherichia coli | |
3.4.13.18 | Mn2+ | when substrates are cleaved rapidly by dipeptidase, addition of Mn2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.18 | Co2+ | when substrates are cleaved rapidly by dipeptidase, addition of Co2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect, activation is not accompanied by change in Km | Escherichia coli | |
3.4.13.18 | Co2+ | enzyme contains two binding sites | Escherichia coli | |
3.4.13.18 | Mn2+ | when substrates are cleaved rapidly by dipeptidase, addition of Mn2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect, activation is accompanied by an increase in Km | Escherichia coli | |
3.4.13.18 | Mn2+ | two binding sites, at least one of which is distinct from a Co2+ site | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.13.18 | 53000 | - |
2 * 53000, SDS-PAGE | Escherichia coli |
3.4.13.18 | 100000 | - |
gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.13.18 | Escherichia coli | - |
- |
- |
3.4.13.18 | Escherichia coli B / ATCC 11303 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.13.18 | strain B | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.13.18 | 760 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.13.18 | -30°C, pH 7.5-8.5, 0.25 M sucrose, stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.13.18 | L-Ala-Gly + H2O | - |
Escherichia coli | L-Ala + Gly | - |
? | |
3.4.13.18 | L-Ala-Gly + H2O | - |
Escherichia coli B / ATCC 11303 | L-Ala + Gly | - |
? | |
3.4.13.18 | additional information | - |
Escherichia coli | ? | - |
? | |
3.4.13.18 | additional information | - |
Escherichia coli B / ATCC 11303 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.13.18 | dimer | 2 * 53000, SDS-PAGE | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.13.18 | 40 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.13.18 | 30 | - |
30 min: stable, 24 h: 40% loss of activity | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.13.18 | 8 | - |
- |
Escherichia coli |