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Literature summary extracted from

  • Minotto, L.; Ko, G.A.; Edwards, M.R.; Bagnara, A.S.
    Trichomonas vaginalis: Expression and characterization of recombinant S-adenosylhomocysteinase (1998), Exp. Parasitol., 90, 175-180.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.13.2.1 medicine antiviral drug design Trichomonas vaginalis
3.13.2.1 medicine anti-parasitic drug design Trichomonas vaginalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.1 into Escherichia coli Trichomonas vaginalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 arabinosyladenine
-
 Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.005 0.01 adenosine native enzyme Trichomonas vaginalis
3.13.2.1 0.02 0.025 adenosine recombinant enzyme Trichomonas vaginalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 55000
-
 gel filtration and SDS-PAGE Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Trichomonas vaginalis
-
 adenosine + L-homocysteine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Trichomonas vaginalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1
-
 Trichomonas vaginalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 0.32
-
 recombinant enzyme Trichomonas vaginalis

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -20°C, in 50% glycerol, stable for at least 1 month Trichomonas vaginalis
3.13.2.1 -70°C, in 50% glycerol, stable for at least 1 month Trichomonas vaginalis
3.13.2.1 4°C, stable for at least 1 month Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Trichomonas vaginalis adenosine + L-homocysteine
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 monomer monomer is enzymatically active, 1 * 55000, SDS-PAGE Trichomonas vaginalis