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Literature summary extracted from

  • Kajander, E.O.; Raina, A.M.
    Affinity-chromatographic purification of S-adenosyl-L-homocysteine hydrolase. Some properties of the enzyme from rat liver (1981), Biochem. J., 193, 503-512.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.13.2.1 freezing and thawing in absence of glycerol causes formation of multimers, low thiol concentrations and exposure to atmospheric O2 fortifies aggregate formation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.1 8-(3-aminopropylamino)adenosine
-
 Rattus norvegicus
3.13.2.1 adenosine irreversible Rattus norvegicus
3.13.2.1 S-adenosyl-L-homocysteine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.0006
-
 adenosine
-
 Rattus norvegicus
3.13.2.1 0.0009
-
 S-adenosyl-L-homocysteine
-
 Rattus norvegicus
3.13.2.1 0.06
-
 L-homocysteine
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 47000
-
 x * 47000, SDS-PAGE Rattus norvegicus
3.13.2.1 210000
-
 gel filtration Rattus norvegicus
3.13.2.1 220000 229000 gel electrophoresis Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O Rattus norvegicus
-
 adenosine + L-homocysteine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Rattus norvegicus
-
 Wistar rats
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.13.2.1 2-mercaptoethanol prevents aggregation of enzyme Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.1 135fold to homogeneity Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.2.1 S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.13.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 5.95
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.13.2.1 -80°C, with 20% glycerol, stable for 8 months Rattus norvegicus
3.13.2.1 0°C, stable in 100 mM potassium phosphate containing 5 mM dithiothreitol, with 1 mM dithiothreitol the enzyme is stable for 1 month but losing activity thereafter rapidly Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 S-adenosyl-L-homocysteine + H2O
-
 Rattus norvegicus adenosine + L-homocysteine
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 ? x * 47000, SDS-PAGE Rattus norvegicus