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Literature summary extracted from
- Comparison of some physicochemical and kinetic properties of S-adenosylhomocysteine hydrolase from bovine liver, bovine adrenal cortex and mouse liver (1982), Biochim. Biophys. Acta, 708, 185-193.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.0002 | - |
adenosine | - |
Mus musculus |  |
| 3.13.2.1 | 0.0002 | - |
adenosine | - |
Bos taurus | |
| 3.13.2.1 | 0.00075 | - |
S-adenosyl-L-homocysteine | - |
Mus musculus | |
| 3.13.2.1 | 0.00075 | - |
S-adenosyl-L-homocysteine | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 46000 | - |
2 * 46000 + 2 * 46000, SDS-PAGE | Mus musculus |
| 3.13.2.1 | 50000 | - |
2 * 50000 + 2 * 50000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Mus musculus | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Bos taurus | - |
adenosine + L-homocysteine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Bos taurus | - |
- |
- |
| 3.13.2.1 | Mus musculus | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 3.13.2.1 | reducing agent such as 2-mercaptoethanol essential for maintenance of catalytic activity | Mus musculus |
| 3.13.2.1 | reducing agent such as 2-mercaptoethanol essential for maintenance of catalytic activity | Bos taurus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | to homogeneity | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | adrenal cortex | - |
Bos taurus | - |
| 3.13.2.1 | liver | - |
Mus musculus | - |
| 3.13.2.1 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 0.3 | 0.9 | - |
Mus musculus |
| 3.13.2.1 | 0.3 | 0.9 | - |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.13.2.1 | -18°C, progressive inactivation especially in absence of reducing agents as e.g. 2-mercaptoethanol | Mus musculus |
| 3.13.2.1 | -18°C, progressive inactivation especially in absence of reducing agents as e.g. 2-mercaptoethanol | Bos taurus |
| 3.13.2.1 | -80°C, storage in liquid nitrogen also possible, 2-20 mg enzyme per ml, stable for at least 1 year | Mus musculus |
| 3.13.2.1 | -80°C, storage in liquid nitrogen also possible, 2-20 mg enzyme per ml, stable for at least 1 year | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Mus musculus | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Bos taurus | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Mus musculus | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Bos taurus | adenosine + L-homocysteine | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | tetramer | 2 * 50000 + 2 * 50000, SDS-PAGE | Bos taurus |
| 3.13.2.1 | tetramer | 2 * 46000 + 2 * 46000, SDS-PAGE | Mus musculus |
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