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Literature summary extracted from
- Occurrence of S-adenosylhomocysteine hydrolase in prokaryote cells. Characterization of the enzyme from Alcaligenes faecalis and role of the enzyme in the activated methyl cycle (1984), Eur. J. Biochem., 141, 385-392.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | adenosine | protection against inactivation by arabinosyladenine | Alcaligenes faecalis |  |
| 3.13.2.1 | homocysteine | protection against inactivation by arabinosyl adenine | Alcaligenes faecalis | |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | - |
Alcaligenes faecalis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | 1-methyladenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 2'-deoxyadenosine | - |
Acinetobacter calcoaceticus | |
| 3.13.2.1 | 2'-deoxyadenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 2-chloroadenosine | - |
Acinetobacter calcoaceticus | |
| 3.13.2.1 | 2-chloroadenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 5'-deoxyadenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 7-deaza-8-azaadenosine | formycin A | Alcaligenes faecalis | |
| 3.13.2.1 | adenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | adenosine 5'-carboxylate | competitive | Alcaligenes faecalis | |
| 3.13.2.1 | adenosine N1-oxide | - |
Alcaligenes faecalis | |
| 3.13.2.1 | arabinosyladenine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | arabinosylguanine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | arabinosylhypoxanthine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | formycin B | - |
Alcaligenes faecalis | |
| 3.13.2.1 | guanosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | Inosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | N6-methyladenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | nebularine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | tubercidin | - |
Alcaligenes faecalis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.014 | - |
S-adenosyl-L-homocysteine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 0.05 | - |
adenosine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 0.25 | - |
L-homocysteine | - |
Alcaligenes faecalis | |
| 3.13.2.1 | 1.07 | - |
DL-homocysteine | - |
Alcaligenes faecalis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 48000 | - |
6 * 48000, SDS-PAGE | Alcaligenes faecalis |
| 3.13.2.1 | 280000 | - |
sedimentation equilibrium | Alcaligenes faecalis |
| 3.13.2.1 | 290000 | - |
gel filtration | Alcaligenes faecalis |
| 3.13.2.1 | 310000 | - |
native PAGE | Alcaligenes faecalis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Alcaligenes faecalis | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Pseudomonas sp. | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Acinetobacter calcoaceticus | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Mycobacterium avium | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Arthrobacter globiformis | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Nocardia asteroides | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Nonomuraea angiospora | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Streptosporangium roseum | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Streptomyces netropsis | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Streptomyces hygroscopicus | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Micromonospora faeni | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Streptomyces yerevanensis | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Alcaligenes faecalis AKU 101 | - |
adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Alcaligenes faecalis IAM 1061 | - |
adenosine + L-homocysteine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Acinetobacter calcoaceticus | - |
IFO 12552 | - |
| 3.13.2.1 | Alcaligenes faecalis | - |
IAM 1061 | - |
| 3.13.2.1 | Alcaligenes faecalis | - |
AKU 101 | - |
| 3.13.2.1 | Alcaligenes faecalis AKU 101 | - |
AKU 101 | - |
| 3.13.2.1 | Alcaligenes faecalis IAM 1061 | - |
IAM 1061 | - |
| 3.13.2.1 | Arthrobacter globiformis | - |
- |
- |
| 3.13.2.1 | Micromonospora faeni | - |
- |
- |
| 3.13.2.1 | Mycobacterium avium | - |
- |
- |
| 3.13.2.1 | Nocardia asteroides | - |
- |
- |
| 3.13.2.1 | Nonomuraea angiospora | - |
- |
- |
| 3.13.2.1 | Pseudomonas sp. | - |
P. putida | - |
| 3.13.2.1 | Pseudomonas sp. | - |
P. aeruginosa | - |
| 3.13.2.1 | Streptomyces hygroscopicus | - |
- |
- |
| 3.13.2.1 | Streptomyces netropsis | - |
- |
- |
| 3.13.2.1 | Streptomyces yerevanensis | - |
- |
- |
| 3.13.2.1 | Streptosporangium roseum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | 39.2fold to homogeneity | Alcaligenes faecalis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 0.00121 | - |
P. aeruginosa, hydrolysis of S-adenosyl-L-homocysteine, crude extract | Pseudomonas sp. |
| 3.13.2.1 | 0.0013 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Arthrobacter globiformis |
| 3.13.2.1 | 0.00133 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Nocardia asteroides |
| 3.13.2.1 | 0.00172 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Acinetobacter calcoaceticus |
| 3.13.2.1 | 0.00215 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Mycobacterium avium |
| 3.13.2.1 | 0.00333 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Streptomyces netropsis |
| 3.13.2.1 | 0.00411 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Streptomyces yerevanensis |
| 3.13.2.1 | 0.00431 | - |
P. putida, hydrolysis of S-adenosyl-L-homocysteine, crude extract | Pseudomonas sp. |
| 3.13.2.1 | 0.00521 | - |
IAM 1061, hydrolysis of S-adenosyl-L-homocysteine, crude extract | Alcaligenes faecalis |
| 3.13.2.1 | 0.00651 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Micromonospora faeni |
| 3.13.2.1 | 0.00731 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Streptomyces hygroscopicus |
| 3.13.2.1 | 0.00855 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Mycobacterium avium |
| 3.13.2.1 | 0.00857 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Streptosporangium roseum |
| 3.13.2.1 | 0.00931 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Arthrobacter globiformis |
| 3.13.2.1 | 0.00938 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Nocardia asteroides |
| 3.13.2.1 | 0.0105 | - |
P. aeruginosa, synthesis of S-adenosyl-L-homocysteine, crude extract | Pseudomonas sp. |
| 3.13.2.1 | 0.0163 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Acinetobacter calcoaceticus |
| 3.13.2.1 | 0.027 | - |
AKU 101, hydrolysis of S-adenosyl-L-homocysteine, crude extract | Alcaligenes faecalis |
| 3.13.2.1 | 0.0324 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Streptomyces netropsis |
| 3.13.2.1 | 0.034 | - |
IAM 1061, synthesis of S-adenosyl-L-homocysteine, crude extract | Alcaligenes faecalis |
| 3.13.2.1 | 0.0358 | - |
hydrolysis of S-adenosyl-L-homocysteine, crude extract | Nonomuraea angiospora |
| 3.13.2.1 | 0.0384 | - |
P. putida, synthesis of S-adenosyl-L-homocysteine, crude extract | Pseudomonas sp. |
| 3.13.2.1 | 0.0492 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Streptomyces yerevanensis |
| 3.13.2.1 | 0.0513 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Streptomyces hygroscopicus |
| 3.13.2.1 | 0.0531 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Micromonospora faeni |
| 3.13.2.1 | 0.0747 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Streptosporangium roseum |
| 3.13.2.1 | 0.0982 | - |
AKU 101, synthesis of S-adenosyl-L-homocysteine, crude extract | Alcaligenes faecalis |
| 3.13.2.1 | 0.375 | - |
synthesis of S-adenosyl-L-homocysteine, crude extract | Nonomuraea angiospora |
| 3.13.2.1 | 3.61 | - |
AKU 101, purified enzyme | Alcaligenes faecalis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | 1-methyladenosine | - |
Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | 2-chloroadenosine + homocysteine | - |
Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | 7-deaza-8-azaadenosine + homocysteine | formycin A | Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | 7-deaza-8-azaadenosine + homocysteine | formycin A | Alcaligenes faecalis AKU 101 | ? | - |
r | |
| 3.13.2.1 | 7-deaza-8-azaadenosine + homocysteine | formycin A | Alcaligenes faecalis IAM 1061 | ? | - |
r | |
| 3.13.2.1 | adenosine N1-oxide + homocysteine | - |
Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | adenosine N1-oxide + homocysteine | - |
Alcaligenes faecalis AKU 101 | ? | - |
r | |
| 3.13.2.1 | adenosine N1-oxide + homocysteine | - |
Alcaligenes faecalis IAM 1061 | ? | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Alcaligenes faecalis | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Pseudomonas sp. | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Acinetobacter calcoaceticus | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Mycobacterium avium | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Arthrobacter globiformis | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Nocardia asteroides | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Nonomuraea angiospora | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Streptosporangium roseum | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Streptomyces netropsis | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Streptomyces hygroscopicus | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Micromonospora faeni | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Streptomyces yerevanensis | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | inosine + homocysteine | - |
Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | N6-methyladenosine + homocysteine | - |
Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | N6-methyladenosine + homocysteine | - |
Alcaligenes faecalis AKU 101 | ? | - |
r | |
| 3.13.2.1 | N6-methyladenosine + homocysteine | - |
Alcaligenes faecalis IAM 1061 | ? | - |
r | |
| 3.13.2.1 | nebularine + homocysteine | - |
Alcaligenes faecalis | ? | - |
r | |
| 3.13.2.1 | nebularine + homocysteine | - |
Alcaligenes faecalis AKU 101 | ? | - |
r | |
| 3.13.2.1 | nebularine + homocysteine | - |
Alcaligenes faecalis IAM 1061 | ? | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Alcaligenes faecalis | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Pseudomonas sp. | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Acinetobacter calcoaceticus | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Mycobacterium avium | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Arthrobacter globiformis | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Nocardia asteroides | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Nonomuraea angiospora | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Streptosporangium roseum | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Streptomyces netropsis | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Streptomyces hygroscopicus | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Micromonospora faeni | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Streptomyces yerevanensis | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Alcaligenes faecalis AKU 101 | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Alcaligenes faecalis IAM 1061 | adenosine + L-homocysteine | - |
r | |
| 3.13.2.1 | tubercidin + L-homocysteine | - |
Alcaligenes faecalis | ? | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | hexamer | - |
Acinetobacter calcoaceticus |
| 3.13.2.1 | hexamer | 6 * 48000, SDS-PAGE | Alcaligenes faecalis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 50 | - |
- |
Alcaligenes faecalis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 37 | - |
at pH 4.0 for 10 min, complete inactivation | Alcaligenes faecalis |
| 3.13.2.1 | 55 | - |
at pH 6.0 for 30 min, half-maximal activity | Alcaligenes faecalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 8 | 10.5 | synthesis and hydrolysis of S-adenosyl-L-homocysteine | Alcaligenes faecalis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 6 | - |
at 55°C for 30 min, half-maximal activity | Alcaligenes faecalis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 4 | - |
at 37°C for 10 min, complete inactivation | Alcaligenes faecalis |
| 3.13.2.1 | 6 | 10 | - |
Alcaligenes faecalis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | NAD+ | - |
Alcaligenes faecalis | |
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