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Literature summary extracted from
- Adenosylhomocysteinase from Yellow lupine (1987), Methods Enzymol., 143, 430-434.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.13.2.1 | purified enzyme tolerates repeated freeze-thawing | Lupinus luteus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | (+/-)-aristeromycin | competitive | Lupinus luteus |  |
| 3.13.2.1 | 2-aza-3-deazaadenosine | competitive | Lupinus luteus | |
| 3.13.2.1 | 3-deazaadenosine | competitive | Lupinus luteus | |
| 3.13.2.1 | 7-deaza-8-azaadenosine | competitive; formycin A | Lupinus luteus | |
| 3.13.2.1 | 8-Aminoadenosine | competitive | Lupinus luteus | |
| 3.13.2.1 | 8-azaadenosine | competitive | Lupinus luteus | |
| 3.13.2.1 | adenosine N1-oxide | competitive | Lupinus luteus | |
| 3.13.2.1 | Inosine | competitive | Lupinus luteus | |
| 3.13.2.1 | N6-methyladenosine | competitive | Lupinus luteus | |
| 3.13.2.1 | nebularine | competitive | Lupinus luteus | |
| 3.13.2.1 | Pyrazomycin | competitive | Lupinus luteus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.0023 | - |
adenosine | - |
Lupinus luteus | |
| 3.13.2.1 | 0.012 | 0.02 | S-adenosyl-L-homocysteine | - |
Lupinus luteus | |
| 3.13.2.1 | 0.13 | - |
L-homocysteine | - |
Lupinus luteus | |
| 3.13.2.1 | 35 | - |
cysteine | - |
Lupinus luteus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 55000 | - |
SDS-PAGE | Lupinus luteus |
| 3.13.2.1 | 110000 | - |
gel filtration | Lupinus luteus |
| 3.13.2.1 | 165000 | - |
pore gradient gel electrophoresis | Lupinus luteus |
| 3.13.2.1 | 200000 | - |
pore gradient gel electrophoresis | Lupinus luteus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Lupinus luteus | - |
adenosine + L-homocysteine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Lupinus luteus | - |
Yellow lupine | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | to homogeneity | Lupinus luteus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine | reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine | Lupinus luteus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | seed | - |
Lupinus luteus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 3.7 | - |
- |
Lupinus luteus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.13.2.1 | -20°C, purified enzyme | Lupinus luteus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | (+/-)-aristeromycin + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 2-aminoadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 2-aza-3-deazaadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 2-chloroadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 2-hydroxyadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 3-deaza-(+/-)-aristeromycin + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 3-deazaadenosine + L-homocysteine | - |
Lupinus luteus | 3-deazaadenosylhomocysteine | - |
r | |
| 3.13.2.1 | 7-deaza-8-azaadenosine + homocysteine | formycin A | Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 8-aminoadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | 8-azaadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | adenosine N1-oxide + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | cysteine + adenosine | - |
Lupinus luteus | AdoCys | - |
r | |
| 3.13.2.1 | DL-homocysteine + adenosine | - |
Lupinus luteus | S-adenosyl-DL-homocysteine | - |
r | |
| 3.13.2.1 | inosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | N6-hydroxyadenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | N6-methyladenosine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | nebularine + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | pyrazomycin + homocysteine | - |
Lupinus luteus | ? | - |
r | |
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Lupinus luteus | adenosine + L-homocysteine | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | dimer | homodimer, 2 * 55000, pore gradient gel electrophoresis | Lupinus luteus |
| 3.13.2.1 | monomer | 1 * 55000, pore gradient gel electrophoresis | Lupinus luteus |
| 3.13.2.1 | tetramer | 4 * 55000 | Lupinus luteus |
| 3.13.2.1 | trimer | homotrimer, 3 * 55000, pore gradient gel electrophoresis | Lupinus luteus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.11 | - |
cysteine | - |
Lupinus luteus | |
| 3.13.2.1 | 4.3 | - |
adenosine | - |
Lupinus luteus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 8.5 | 9 | - |
Lupinus luteus |
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