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Literature summary extracted from

  • Terada, M.; Tatibana, M.; Hayaishi, O.
    Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens (1967), J. Biol. Chem., 242, 5578-5585.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.8 adenosine at 6.25 mM, competitive inhibitor for uridine hydrolysis Pseudomonas fluorescens
3.2.2.8 AgCl at 1 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 CaCl2 at 1 mM, inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 CdCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 CoCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 CuCl2 at 1 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 cytidine at 6.25 mM, competitive inhibitor for uridine hydrolysis Pseudomonas fluorescens
3.2.2.8 EDTA at 1 mM: inhibits uridine hydrolysis by 50% Pseudomonas fluorescens
3.2.2.8 FeCl3 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 FeSO4 at 1 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 HgCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 Inosine at 6.25 mM, competitive inhibitor for uridine hydrolysis Pseudomonas fluorescens
3.2.2.8 MgCl2 at 1 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 MnSO4 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 NH4Cl at 1 mM: inhibition less than 10% Pseudomonas fluorescens
3.2.2.8 ZnCl2 at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10% Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.8 0.2
-
 Inosine pH 8.5 Pseudomonas fluorescens
3.2.2.8 0.5
-
 Inosine
-
 Pseudomonas fluorescens
3.2.2.8 0.5
-
 adenosine pH 8.5 Pseudomonas fluorescens
3.2.2.8 0.6
-
 guanosine
-
 Pseudomonas fluorescens
3.2.2.8 0.8
-
 uridine
-
 Pseudomonas fluorescens
3.2.2.8 1
-
 cytidine pH 8.5 Pseudomonas fluorescens
3.2.2.8 2.5
-
 5-Bromouridine
-
 Pseudomonas fluorescens
3.2.2.8 6.5
-
 Xanthosine
-
 Pseudomonas fluorescens
3.2.2.8 7.5
-
 1-beta-D-ribofuranosylthymine
-
 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.8 N-D-ribosylpyrimidine Pseudomonas fluorescens
-
 pyrimidine + D-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.8 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.8 partial purification, 300fold Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.8 272
-
 at 37°C Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.2.2.8 -15°C, 1-3 mg of protein per ml, stable for 6 months Pseudomonas fluorescens
3.2.2.8 4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.8 1-beta-D-ribofuranosylnicotinamide + H2O
-
 Pseudomonas fluorescens nicotinamide + D-ribose
-
 ?
3.2.2.8 1-beta-D-ribofuranosylthymine + H2O
-
 Pseudomonas fluorescens D-ribose + thymine
-
 ?
3.2.2.8 4-amino-5-imidazolecarboxamide ribonucleotide + H2O
-
 Pseudomonas fluorescens 4-amino-5-imidazolecarboxamide + D-ribose
-
 ?
3.2.2.8 5-bromouridine + H2O
-
 Pseudomonas fluorescens 5-bromouracil + D-ribose
-
 ?
3.2.2.8 6-azauridine + H2O poor substrate Pseudomonas fluorescens 6-azauracil + D-ribose
-
 ?
3.2.2.8 adenosine + H2O
-
 Pseudomonas fluorescens adenine + D-ribose
-
 ?
3.2.2.8 cytidine + H2O
-
 Pseudomonas fluorescens cytosine + D-ribose
-
 ?
3.2.2.8 guanosine + H2O
-
 Pseudomonas fluorescens guanine + D-ribose
-
 ?
3.2.2.8 imidazoleacetic acid ribonucleotide + H2O
-
 Pseudomonas fluorescens imidazoleacetic acid + D-ribose
-
 ?
3.2.2.8 inosine + H2O
-
 Pseudomonas fluorescens hypoxanthine + D-ribose
-
 ?
3.2.2.8 N-D-ribosylpyrimidine
-
 Pseudomonas fluorescens pyrimidine + D-ribose
-
 ?
3.2.2.8 N-D-ribosylpyrimidine + H2O
-
 Pseudomonas fluorescens pyrimidine + D-ribose
-
 ?
3.2.2.8 purine D-ribonucleosides + H2O prefers pyrimidines Pseudomonas fluorescens purine + D-ribose
-
 ?
3.2.2.8 tubercidin + H2O poor substrate Pseudomonas fluorescens 1-deazaadenine + D-ribose
-
 ?
3.2.2.8 uridine + H2O
-
 Pseudomonas fluorescens uracil + D-ribose
-
 ?
3.2.2.8 xanthosine + H2O
-
 Pseudomonas fluorescens xanthine + D-ribose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.8 45
-
 for 10 min at pH 7.0-9.2, more than 90% of activity remains Pseudomonas fluorescens
3.2.2.8 45
-
 for 10 min at pH 5.0, 50% of activity remains Pseudomonas fluorescens
3.2.2.8 60
-
 for 15 min, 60% denaturation Pseudomonas fluorescens
3.2.2.8 70
-
 for 5 min at pH 8.0, complete inactivation Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.8 6
-
 substrate: inosine Pseudomonas fluorescens
3.2.2.8 6.5
-
 substrate: guanosine Pseudomonas fluorescens
3.2.2.8 7
-
 substrate: xanthosine Pseudomonas fluorescens
3.2.2.8 8
-
 substrate: 1-beta-D-ribofuranosylthymine Pseudomonas fluorescens
3.2.2.8 8.5
-
 substrate: cytidine, uridine, 5-bromouridine and adenosine Pseudomonas fluorescens