BRENDA - Enzyme Database

An adenosine hydrolase from Brussels sprouts

Mazelis, M.; Creveling, R.K.; J. Biol. Chem. 238, 3358-3361 (1963)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.2.7
stable against repeated freezing and thawing cycles
Brassica oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.7
adenine
38% inhibition at 9 mM
Brassica oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.7
0.002
-
adenosine
-
Brassica oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.7
2'-deoxyadenosine + H2O
Brassica oleracea
hydrolysis at the same rate as for adenosine
6-aminopurine + 2-deoxy-D-ribofuranose
-
Brassica oleracea
?
3.2.2.7
2'-deoxyadenosine + H2O
Brassica oleracea
6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside
6-aminopurine + 2-deoxy-D-ribofuranose
-
Brassica oleracea
?
3.2.2.7
adenosine + H2O
Brassica oleracea
6-aminopurine-9-beta-D-ribofuranoside
6-aminopurine + D-ribofuranose
-
Brassica oleracea
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.7
Brassica oleracea
-
var. gemmifera
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.7
partial
Brassica oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.7
sprout
-
Brassica oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.7
0.62
-
-
Brassica oleracea
Storage Stability
EC Number
Storage Stability
Organism
3.2.2.7
-10°C, 10 mM NaHCO3, 4 months
Brassica oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.7
2'-deoxyadenosine + H2O
hydrolysis at the same rate as for adenosine
137113
Brassica oleracea
6-aminopurine + 2-deoxy-D-ribofuranose
-
137113
Brassica oleracea
?
3.2.2.7
2'-deoxyadenosine + H2O
6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside
137113
Brassica oleracea
6-aminopurine + 2-deoxy-D-ribofuranose
-
137113
Brassica oleracea
?
3.2.2.7
adenosine + H2O
6-aminopurine-9-beta-D-ribofuranoside
137113
Brassica oleracea
6-aminopurine + D-ribofuranose
-
137113
Brassica oleracea
ir
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.7
10
-
stable for 15 days
Brassica oleracea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.7
3.5
4.5
optimum activity varies with buffer used
Brassica oleracea
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.2.7
stable against repeated freezing and thawing cycles
Brassica oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.7
adenine
38% inhibition at 9 mM
Brassica oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.7
0.002
-
adenosine
-
Brassica oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.7
2'-deoxyadenosine + H2O
Brassica oleracea
hydrolysis at the same rate as for adenosine
6-aminopurine + 2-deoxy-D-ribofuranose
-
Brassica oleracea
?
3.2.2.7
2'-deoxyadenosine + H2O
Brassica oleracea
6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside
6-aminopurine + 2-deoxy-D-ribofuranose
-
Brassica oleracea
?
3.2.2.7
adenosine + H2O
Brassica oleracea
6-aminopurine-9-beta-D-ribofuranoside
6-aminopurine + D-ribofuranose
-
Brassica oleracea
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.7
partial
Brassica oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.7
sprout
-
Brassica oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.7
0.62
-
-
Brassica oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.2.7
-10°C, 10 mM NaHCO3, 4 months
Brassica oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.7
2'-deoxyadenosine + H2O
hydrolysis at the same rate as for adenosine
137113
Brassica oleracea
6-aminopurine + 2-deoxy-D-ribofuranose
-
137113
Brassica oleracea
?
3.2.2.7
2'-deoxyadenosine + H2O
6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside
137113
Brassica oleracea
6-aminopurine + 2-deoxy-D-ribofuranose
-
137113
Brassica oleracea
?
3.2.2.7
adenosine + H2O
6-aminopurine-9-beta-D-ribofuranoside
137113
Brassica oleracea
6-aminopurine + D-ribofuranose
-
137113
Brassica oleracea
ir
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.7
10
-
stable for 15 days
Brassica oleracea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.7
3.5
4.5
optimum activity varies with buffer used
Brassica oleracea