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Literature summary extracted from

  • Mazelis, M.; Creveling, R.K.
    An adenosine hydrolase from Brussels sprouts (1963), J. Biol. Chem., 238, 3358-3361.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.7 stable against repeated freezing and thawing cycles Brassica oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.7 adenine 38% inhibition at 9 mM Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.7 0.002
-
 adenosine
-
 Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.7 2'-deoxyadenosine + H2O Brassica oleracea hydrolysis at the same rate as for adenosine 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 2'-deoxyadenosine + H2O Brassica oleracea 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O Brassica oleracea 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.7 Brassica oleracea
-
 var. gemmifera
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.7 partial Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.7 sprout
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.7 0.62
-
-
 Brassica oleracea

Storage Stability

EC Number Storage Stability Organism
3.2.2.7 -10°C, 10 mM NaHCO3, 4 months Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.7 2'-deoxyadenosine + H2O hydrolysis at the same rate as for adenosine Brassica oleracea 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Brassica oleracea 6-aminopurine + 2-deoxy-D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Brassica oleracea 6-aminopurine + D-ribofuranose
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.7 10
-
 stable for 15 days Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.7 3.5 4.5 optimum activity varies with buffer used Brassica oleracea