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Literature summary extracted from

  • Anderson, B.M.; Yuan, J.H.
    NAD glycohydrolase from bovine seminal plasma (1986), Methods Enzymol., 66, 144-150.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.5 adenosine
-
 Bos taurus
3.2.2.5 ADP
-
 Bos taurus
3.2.2.5 ADPribose
-
 Bos taurus
3.2.2.5 AMP
-
 Bos taurus
3.2.2.5 additional information N1-alkylnicotinamide chlorides, n-alkylphosphates, aliphatic carboxylic acids, other pyridine bases other than nicotinamide, certain pyridine dinucleotides Bos taurus
3.2.2.5 NADH
-
 Bos taurus
3.2.2.5 nicotinamide
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.5 0.006
-
 3-acetylpyridine adenine dinucleotide 25°C Bos taurus
3.2.2.5 0.1
-
 NAD+ 25°C , seminal fluid Bos taurus
3.2.2.5 0.32
-
 NAD+ 38°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.5 36300
-
 sedimentation equilibrium studies Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.5 NAD+ + H2O Bos taurus
-
 ADPribose + nicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.5 Bos taurus
-
 bull
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.2.5 glycoprotein 10.4% carbohydrate Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.5
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.5 semen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.5 9900
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.2.5 -15°C, potassium phosphate buffer, pH 7.4, 2 months Bos taurus
3.2.2.5 4°C, potassium phosphate buffer, pH 7.4, loss of 40% of activity in 4 weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.5 3-acetylpyridine adenine dinucleotide + H2O
-
 Bos taurus ?
-
 ?
3.2.2.5 3-acetylpyridine hypoxanthine dinucleotide + H2O
-
 Bos taurus ?
-
 ?
3.2.2.5 NAD+ + H2O
-
 Bos taurus ADPribose + nicotinamide
-
 ?
3.2.2.5 NADP+ + H2O
-
 Bos taurus phospho-ADPribose + nicotinamide
-
 ?
3.2.2.5 nicotinamide hypoxanthine dinucleotide + H2O
-
 Bos taurus ?
-
 ?
3.2.2.5 NMN + H2O
-
 Bos taurus phosphoribose + nicotinamide
-
 ?
3.2.2.5 pyridine-3-aldehyde adenine dinucleotide + H2O
-
 Bos taurus ?
-
 ?
3.2.2.5 pyridine-3-aldehyde hypoxanthine dinucleotide + H2O
-
 Bos taurus ?
-
 ?
3.2.2.5 thioNAD+ + H2O
-
 Bos taurus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.5 50
-
 at or below no loss of activity for 1 h Bos taurus
3.2.2.5 100
-
 5 min, complete inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.5 7.5
-
-
 Bos taurus