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Literature summary extracted from

  • Maehama, T.; Nishina, H.; Katada, T.
    ADP-ribosylarginine glycohydrolase catalyzing the release of ADP-ribose from the cholera toxin-modified alpha-subunits of GTP-binding proteins (1994), J. Biochem., 116, 1134-1138.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.19 dithiothreitol as a GST-fusion protein Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.19 expression as a glutathione S-transferase-linked fusion protein in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.19 ADP inhibition with 1 mM ADP Rattus norvegicus
3.2.2.19 ADP-ribosylarginine methyl ester
-
Rattus norvegicus
3.2.2.19 ADPribose inhibition with 1 mM ADP-ribose Rattus norvegicus
3.2.2.19 alphaNAD+ inhibition with 1 mM alphaNAD+ Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.19 0.005
-
ADP-ribosylarginine methyl ester as a GST-fusion protein Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.19 Mg2+ as a GST-fusion protein Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.19 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.19 as a GST-fusion protein almost to homogeneity by affinity-column chromatography of glutathione-Sepharose Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.19 ADP-ribosylarginine methyl ester + H2O
-
Rattus norvegicus ADP-ribose + arginine methyl ester
-
?
3.2.2.19 alpha-subunit of stimulating GTP-binding protein ADP-ribosylated + H2O the GDP-bound form of ADP-ribosylated stimulating GTP-binding protein-alpha is more rapidly hydrolyzed than the GTPgammaS-bound form Rattus norvegicus alpha-subunit of stimulating GTP-binding protein + ADP-ribose
-
?
3.2.2.19 cholera toxin A1-subunit ADP-ribosylated + H2O
-
Rattus norvegicus cholera toxin A1-subunit + ADP-ribose
-
?
3.2.2.19 nonmuscle actin ADP-ribosylated + H2O
-
Rattus norvegicus nonmuscle actin + ADP-ribose
-
?