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Literature summary extracted from

  • Anderson, R.L.; Palmer, R.E.
    Phosphocellobiase (1975), Methods Enzymol., 42 C, 494-497.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.23 0.5 Salicin 6-phosphate
-
 Klebsiella aerogenes
3.2.1.86 0.23 0.5 arbutin 6-phosphate
-
 Klebsiella aerogenes
3.2.1.86 0.23 0.5 phenyl beta-D-glucoside 6-phosphate
-
 Klebsiella aerogenes
3.2.1.86 0.23 0.5 cellobiose 6-phosphate
-
 Klebsiella aerogenes
3.2.1.86 0.23 0.5 6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
-
 Klebsiella aerogenes
3.2.1.86 0.23 0.5 cellobiitol monophosphate
-
 Klebsiella aerogenes
3.2.1.86 0.23 0.5 methyl beta-D-glucoside monophosphate
-
 Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.86 52000
-
 sucrose density gradient centrifugation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.86 cellobiose 6-phosphate + H2O Klebsiella aerogenes
-
 D-glucose 6-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.86 14 fold Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.86 0.54
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 arbutin 6-phosphate + H2O
-
 Klebsiella aerogenes hydroquinone + D-glucose 6-phosphate
-
 ?
3.2.1.86 cellobiitol monophosphate + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.1.86 cellobiose 6-phosphate + H2O
-
 Klebsiella aerogenes D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 gentiobiose 6-phosphate + H2O
-
 Klebsiella aerogenes D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 methyl beta-D-glucoside monophosphate + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.1.86 phenyl beta-D-glucoside 6-phosphate + H2O
-
 Klebsiella aerogenes phenol + glucose 6-phosphate
-
 ?
3.2.1.86 salicin 6-phosphate + H2O
-
 Klebsiella aerogenes 2-hydroxymethylphenol + D-glucose 6-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.86 7 8 cellobiose 6-phosphate Klebsiella aerogenes