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Literature summary extracted from
- Glucosidase II from control and ethanol-treated rats: purification and properties (1991), Biol. Chem., 372, 373-380.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.207 | 2-propanol | - |
Rattus norvegicus |  |
| 3.2.1.207 | Ca2+ | - |
Rattus norvegicus | |
| 3.2.1.207 | ethanol | - |
Rattus norvegicus | |
| 3.2.1.207 | isobutanol | - |
Rattus norvegicus | |
| 3.2.1.207 | methanol | - |
Rattus norvegicus | |
| 3.2.1.207 | Mg2+ | - |
Rattus norvegicus | |
| 3.2.1.207 | n-butanol | - |
Rattus norvegicus | |
| 3.2.1.207 | n-Propanol | - |
Rattus norvegicus | |
| 3.2.1.207 | NH4+ | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.207 | 1.2 | - |
p-nitrophenyl alpha-D-glucopyranoside | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.207 | microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 106000 | - |
alpha4, 4 * 106000 | Rattus norvegicus |
| 3.2.1.207 | 107000 | - |
may be a proteolytic degradation product of the 112000 protein | Rattus norvegicus |
| 3.2.1.207 | 425000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.207 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.207 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.207 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 0.617 | - |
p-nitrophenyl-alpha-D-glucopyranoside as substrate | Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.207 | 4°C, 10 mM potassium phosphate buffer, pH 7, 0.1% Triton X-100, 5 mM 2-mercaptoethanol, 14 days | Rattus norvegicus |
| 3.2.1.207 | 4°C, 10 mM potassium phosphate buffer, pH 7, 0.1% Triton X-100, 5 mM 2-mercaptoethanol, 8 days ethanol treated rats | Rattus norvegicus |
| 3.2.1.207 | freezing inactivates the enzyme | Rattus norvegicus |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.207 | tetramer | alpha4, 4 * 106000 | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 40 | - |
100% of activity after 30 min, 10 mM potassium phosphate buffer, pH 7, 0.1% Triton X-100, 5 mM 2-mercaptoethanol | Rattus norvegicus |
| 3.2.1.207 | 47 | - |
47% of activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 6.5 | 7 | 0.75 mM maltose | Rattus norvegicus |
| 3.2.1.207 | 6.8 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 3 | 7.5 | citrate/phosphate buffer | Rattus norvegicus |
| 3.2.1.207 | 6.8 | 8.2 | HEPES buffer | Rattus norvegicus |
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