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Literature summary extracted from

  • Burns, D.M.; Touster, O.
    Purification and characterization of glucosidase II, an endoplasmic reticulum hydrolase involved in glycoprotein biosynthesis (1982), J. Biol. Chem., 257, 9991-10000.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.207 2-deoxy-D-glucose activation substrate p-nitrophenyl-alpha-D-glucoside Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.207 D-glucono-1,5-lactone
-
 Rattus norvegicus
3.2.1.207 glucose
-
 Rattus norvegicus
3.2.1.207 glycerol
-
 Rattus norvegicus
3.2.1.207 maltose
-
 Rattus norvegicus
3.2.1.207 p-chloromercuribenzenesulfonate
-
 Rattus norvegicus
3.2.1.207 Tris
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.207 0.85
-
 p-nitrophenyl alpha-D-glucopyranoside
-
 Rattus norvegicus
3.2.1.207 4.8
-
 maltose
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.207 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.207 260000 290000 gel electrophoresis, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.207 Glc2Man9GlcNAc2 + H2O Rattus norvegicus
-
 Man9GlcNAc2 + alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.207
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.207 Glc2Man9GlcNAc2-[protein] + H2O = GlcMan9GlcNAc2-[protein] + beta-D-glucopyranose Glc2Man9GlcNAc2 + H2O = Man9GlcNAc2 + glucose Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.207 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.207 -70°C, 100 mM phosphate buffer, pH 7, 100 mM maltose, 5 mM mercaptoethanol, several weeks Rattus norvegicus
3.2.1.207 4-6°C, 100 mM phosphate buffer, pH 7, 10% glycerol, 5 mM mercaptoethanol, several days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Rattus norvegicus 4-nitrophenol + D-glucose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + 2 H2O
-
 Rattus norvegicus Man9GlcNAc2 + 2 D-glucose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O
-
 Rattus norvegicus Man9GlcNAc2 + alpha-D-glucose
-
 ?
3.2.1.207 maltose + H2O
-
 Rattus norvegicus alpha-D-glucose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.207 tetramer SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.207 36
-
-
 Rattus norvegicus
3.2.1.207 40
-
 inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.207 6.6 7 depending on substrate Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.207 6 8 with 5 mM mercaptoethanol Rattus norvegicus