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Literature summary extracted from
- beta-Agarase from Pseudomonas sp. W7: purification and recombinant enzyme from Escherichia coli and the effects of salt on its activity (1997), Biotechnol. Appl. Biochem., 26, 1-6.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.81 | expression in Escherichia coli | Vibrio sp. |
| 3.2.1.81 | expression in Escherichia coli | Pseudoalteromonas atlantica |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.81 | NaCl | 0.9 M | Pseudomonas sp. |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 55000 | - |
- |
Pseudoalteromonas atlantica |
| 3.2.1.81 | 59000 | - |
SDS-PAGE | Pseudomonas sp. |
| 3.2.1.81 | 105000 | - |
- |
Vibrio sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.81 | agarose + H2O | Pseudomonas sp. | - |
neoagarotetraose | - |
ir | |
| 3.2.1.81 | agarose + H2O | Vibrio sp. | - |
neoagarotetraose | - |
ir | |
| 3.2.1.81 | agarose + H2O | Pseudoalteromonas atlantica | - |
neoagarotetraose | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.81 | Pseudoalteromonas atlantica | - |
- |
- |
| 3.2.1.81 | Pseudomonas sp. | - |
- |
- |
| 3.2.1.81 | Vibrio sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.81 | - |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 43.3 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.81 | agarose + H2O | - |
Pseudomonas sp. | neoagarotetraose | - |
ir | |
| 3.2.1.81 | agarose + H2O | - |
Vibrio sp. | neoagarotetraose | - |
ir | |
| 3.2.1.81 | agarose + H2O | - |
Pseudoalteromonas atlantica | neoagarotetraose | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 20 | 40 | - |
Pseudomonas sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.81 | 7.8 | - |
- |
Pseudomonas sp. |
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Release 2023.1 (January 2023)
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