Literature summary extracted from

Rixon, J.E.; Ferreira, L.M.A.; Durrant, A.J.; Laurie, J.I.; Hazlewood, G.O.; Gilbert, H.J.
Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa (1992), Biochem. J., 285, 947-955.

No PubMed abstract available

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.74	Escherichia coli	Pseudomonas fluorescens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.74	0.028	-	cellohexaose	-	Pseudomonas fluorescens
3.2.1.74	1.2	-	cellobiose	-	Pseudomonas fluorescens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.2.1.74	cell envelope	expressed in Escherichia coli	Pseudomonas fluorescens	30313	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.2.1.74	80000	-	x * 80000, SDS-PAGE	Pseudomonas fluorescens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.74	Pseudomonas fluorescens	-	subsp. cellulosa	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.74	4-methylumbelliferyl beta-cellobioside + H2O	-	Pseudomonas fluorescens	4-methylumbelliferone + cellobiose	-	?
3.2.1.74	4-methylumbelliferyl beta-D-glucoside + H2O	-	Pseudomonas fluorescens	4-methylumbelliferone + D-glucopyranose	-	?
3.2.1.74	cellobiose + H2O	-	Pseudomonas fluorescens	D-glucose + cellotetraose + cellotriose	D-glucose only	?
3.2.1.74	cellohexaose + H2O	-	Pseudomonas fluorescens	D-glucose + ?	in the early stages also cellopentaose and cellotetraose	?
3.2.1.74	cellopentaose + H2O	-	Pseudomonas fluorescens	D-glucose + ?	-	?
3.2.1.74	cellotetraose + H2O	-	Pseudomonas fluorescens	D-glucose + cellotriose + cellobiose	-	?
3.2.1.74	cellotriose + H2O	-	Pseudomonas fluorescens	D-glucose + cellobiose	D-glucose only	?
3.2.1.74	p-nitrophenyl-beta-D-cellobioside + H2O	-	Pseudomonas fluorescens	D-glucose + p-nitrophenyl-beta-D-glucoside	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.74	?	x * 80000, SDS-PAGE	Pseudomonas fluorescens

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Release 2023.1 (January 2023)