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Literature summary extracted from

  • Heinrichova, K.
    Isolation, characterization and mode of action of exo-D-galacturonanase from carrot (1977), Collect. Czech. Chem. Commun., 42, 3214-3221.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.67 stable, even at high temperatures, relatively stable in acidic and neutral, unstable in alkaline media Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 NaCl
-
 Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 0.423
-
 pectic acid
-
 Daucus carota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 pectic acid + H2O Daucus carota
-
 D-galacturonide + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Daucus carota
-
 carrot
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67
-
 Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 root
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 0.44
-
 2 several forms of exo-D-galacturonanase Daucus carota
3.2.1.67 0.56
-
 2 several forms of exo-D-galacturonanase Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 (1,4-alpha-D-galacturonide)2 + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)4 + H2O
-
 Daucus carota (1,4-alpha-D-galacturonide)3 + D-galacturonic acid
-
 ?
3.2.1.67 (1,4-alpha-D-galacturonide)n + H2O
-
 Daucus carota D-galacturonate + digalacturonic acid + trigalacturonic acid
-
 ?
3.2.1.67 oligo-D-galacturonate + H2O
-
 Daucus carota D-galacturonic acid
-
 ?
3.2.1.67 p-nitrophenyl-alpha-D-galactopyranuronide + H2O cleaves all substrates with 4-deoxy-L-threo-hexo-4-eno-pyranosyluronate unit at the nonreducing end Daucus carota D-galacturonic acid + 4-nitrophenol
-
 ?
3.2.1.67 pectic acid + H2O degraded not fully Daucus carota D-galacturonic acid + ?
-
 ?
3.2.1.67 pectic acid + H2O
-
 Daucus carota D-galacturonide + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 60 70 pectate, optimum temperature for enzyme action Daucus carota

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 60 70 highly stable Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 5.1
-
 pectate, native enzyme Daucus carota