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Literature summary extracted from

  • Lim, Y.S.; Kang, S.K.; Lee, S.O.; Lee, J.D.; Lee, T.H.
    Purification and charakterization of a levanase from Streptomyces sp. 366L (1998), J. Biotechnol., 61, 33-41.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.65 agriculture plant differentiation, growth promoting activities for some enteric bacteria Streptomyces sp. 366L
3.2.1.65 medicine enhancing effect on the immune system and other physiological functions Streptomyces sp. 366L
3.2.1.65 nutrition energy source Streptomyces sp. 366L
3.2.1.65 nutrition production of noncaloric sweeteners Streptomyces sp. 366L
3.2.1.65 synthesis protection from aging Streptomyces sp. 366L

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.65 Ag+
-
Streptomyces sp. 366L
3.2.1.65 Co2+
-
Streptomyces sp. 366L
3.2.1.65 iodoacetate
-
Streptomyces sp. 366L
3.2.1.65 Mn2+
-
Streptomyces sp. 366L
3.2.1.65 p-chloromercuribenzoate
-
Streptomyces sp. 366L
3.2.1.65 SDS
-
Streptomyces sp. 366L
3.2.1.65 Zn2+
-
Streptomyces sp. 366L

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.65 78000
-
SDS-PAGE Streptomyces sp. 366L
3.2.1.65 80000
-
gel filtration Streptomyces sp. 366L

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Streptomyces sp. 366L
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.65
-
Streptomyces sp. 366L

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 4309
-
U/mg Streptomyces sp. 366L

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 levan + H2O
-
Streptomyces sp. 366L levanheptaose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.65 monomer SDS-PAGE Streptomyces sp. 366L
3.2.1.65 monomer gel filtration Streptomyces sp. 366L

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 40
-
rapidly inactivated above 45°C Streptomyces sp. 366L
3.2.1.65 40
-
pH 7.0 Streptomyces sp. 366L

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 7
-
rapidly decreased activity below and above pH 7.0 Streptomyces sp. 366L

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.65 6 10 20°C Streptomyces sp. 366L