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Literature summary extracted from

  • Zhou, J.; Baba, T.; Takano, T.; Kobayashi, S.; Arai, Y.
    Properties of the enzyme expressed by the Pseudomonas saccharophila maltotetrahydrolase gene (mta) in Escherichia coli (1992), Carbohydr. Res., 223, 255-261.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.60 expression in Escherichia coli Pelomonas saccharophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.60 Ag+ AgNO3, recombinant and native enzyme Pelomonas saccharophila
3.2.1.60 Cu2+ CuSO4, recombinant and native enzyme Pelomonas saccharophila
3.2.1.60 Fe3+ FeCl3, recombinant and native enzyme Pelomonas saccharophila
3.2.1.60 Pb2+ Pb(OAc)2, recombinant and native enzyme Pelomonas saccharophila
3.2.1.60 Zn2+
-
 Pelomonas saccharophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.60 56000
-
 recombinant and native enzyme, SDS-PAGE Pelomonas saccharophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.60 Pelomonas saccharophila
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.60 amylopectin + H2O recombinant and native enzyme Pelomonas saccharophila maltotetraose + ?
-
 ?
3.2.1.60 amylose + H2O recombinant and native enzyme Pelomonas saccharophila maltotetraose
-
 ?
3.2.1.60 glycogen + H2O recombinant and native enzyme, oyster and rabbit liver glycogen Pelomonas saccharophila maltotetraose
-
 ?
3.2.1.60 starch + H2O recombinant and native enzyme Pelomonas saccharophila maltotetraose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.60 45
-
 recombinant and native enzyme Pelomonas saccharophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.60 additional information
-
 2 mM CaCl2, thermal stability of the recombinant and native enzymes increase Pelomonas saccharophila
3.2.1.60 40
-
 recombinant and native enzyme stable on heating in 10 mM acetate buffer, pH 6.0, 30 min, 40°C and below Pelomonas saccharophila
3.2.1.60 60
-
 recombinant and native enzyme, complete loss of activity Pelomonas saccharophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.60 6.5
-
 recombinant and native enzyme Pelomonas saccharophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.60 4 9 recombinant and native enzyme, pH 4.0, 30%, pH 9.0, 50% of maximal activity Pelomonas saccharophila