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Literature summary extracted from
- Isolation, purification, and characterization of a maltotetraose-producing amylase from Pseudomonas stutzeri (1971), Arch. Biochem. Biophys., 145, 105-114.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.60 | dialysis, 4°C, prolonged periods, complete loss of activity | Pseudomonas stutzeri |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.60 | extracellular | - |
Pseudomonas stutzeri | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.60 | 12500 | - |
x * 12500, dimers, tetramers, hexamers, octamers or decamers, concentration dependent | Pseudomonas stutzeri |
| 3.2.1.60 | 125000 | - |
gel filtration | Pseudomonas stutzeri |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.60 | Pseudomonas stutzeri | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.60 | - |
Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.60 | 2590 | - |
- |
Pseudomonas stutzeri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.60 | amylopectin + H2O | - |
Pseudomonas stutzeri | maltotetraose + ? | - |
? |  |
| 3.2.1.60 | maltoheptaose + H2O | - |
Pseudomonas stutzeri | maltotetraose + maltotriose | - |
? | |
| 3.2.1.60 | maltohexaose + H2O | - |
Pseudomonas stutzeri | maltotetraose + maltose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.60 | oligomer | x * 12500, dimers, tetramers, hexamers, octamers or decamers, concentration dependent | Pseudomonas stutzeri |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.60 | 47 | - |
- |
Pseudomonas stutzeri |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.60 | 15 | 60 | 15°C, 20% of maximal activity, 60°C, 30% of maximal activity | Pseudomonas stutzeri |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.60 | 8 | - |
- |
Pseudomonas stutzeri |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.60 | 4.5 | 10.5 | 95% of maximum activity at pH 7 and pH 9.5, pH 4.5, 40% of maximal activity, pH 10.5, 82% of maximal activity | Pseudomonas stutzeri |
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Release 2023.1 (January 2023)
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