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Literature summary extracted from

  • Simonson, L.G.; Gaugler, R.W.; Lamberts, B.L.; Reiher, D.A.
    Purification and properties of endo-1, 3-alpha-D-glucanase from Pseudomonas (1982), Biochim. Biophys. Acta, 715, 189-195.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.59 Ag+
-
 Pseudomonas sp.
3.2.1.59 EDTA
-
 Pseudomonas sp.
3.2.1.59 Fe3+
-
 Pseudomonas sp.
3.2.1.59 Hg2+
-
 Pseudomonas sp.
3.2.1.59 p-chloromercuribenzoic acid
-
 Pseudomonas sp.
3.2.1.59 Pb2+
-
 Pseudomonas sp.
3.2.1.59 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.59 80
-
 limit glucan
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.59 Ca2+ increases activity Pseudomonas sp.
3.2.1.59 Co2+ increases activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.59 279000
-
 aggregated form, gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.59 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.59 NRRL-B-12324 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.59 culture supernatant
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.59 78.1
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.59 4°C, 18 months, 17% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.59 limit glucan + H2O
-
 Pseudomonas sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.59 56
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.59 37
-
 pH 5.5, 3 days, 3% loss of activity Pseudomonas sp.
3.2.1.59 60
-
 loss of activity at or above Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.59 5
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.59 4.5 8 sharp decrease below pH 4.5, gradual decrease up to pH 8.0 Pseudomonas sp.