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Literature summary extracted from

  • Kim, T.J.; Shin, J.H.; Oh, J.H.; Kim, M.J.; Lee, S.B.; Ryu, S.; Kwon, K.; Kim, J.W.; Choi, E.H.; Robyt, J.F.; Park, K.H.
    Analysis of the gene encoding cyclomaltodextrinase from alkalophilic Bacillus sp. I-5 and cgaracterization of enzymatic properties (1998), Arch. Biochem. Biophys., 353, 221-227.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.54 EDTA 5 mM EDTA, activity increases more than 2fold, relative activity 237.2% Bacillus sp. (in: Bacteria)
3.2.1.54 EGTA increases activity about 2.3fold, relative activity 236.3% Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 CDase I-5 gene, pBR322, expression in Escherichia coli MC1061 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 Ag+
-
Bacillus sp. (in: Bacteria)
3.2.1.54 Cr3+ relative activity 58.4% Bacillus sp. (in: Bacteria)
3.2.1.54 Cu2+
-
Bacillus sp. (in: Bacteria)
3.2.1.54 Fe3+ relative activity 37.7% Bacillus sp. (in: Bacteria)
3.2.1.54 Li+ relative activity 75.5% Bacillus sp. (in: Bacteria)
3.2.1.54 Mg2+ relative activity 43% Bacillus sp. (in: Bacteria)
3.2.1.54 Mn2+ relative activity 64.2% Bacillus sp. (in: Bacteria)
3.2.1.54 Ni2+ relative activity 72.2% Bacillus sp. (in: Bacteria)
3.2.1.54 Zn2+ relative activity 5.6% Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.54 Ba2+ relative activity 101.5% Bacillus sp. (in: Bacteria)
3.2.1.54 Ca2+ activated and stabilized by Ca2+, relative activity 128.7% Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 63000
-
MW deduced from the nucleotide sequence 64884 Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Bacillus sp. (in: Bacteria)
-
I-5
-
3.2.1.54 Bacillus sp. (in: Bacteria) I-5
-
I-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 gene encoding enzyme cloned in Escherichia coli, expressed and purified from Escherichia coli Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 148
-
crude enzyme, purification 1fold Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 acarbose + H2O pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme Bacillus sp. (in: Bacteria) ?
-
?
3.2.1.54 acarbose + H2O pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme Bacillus sp. (in: Bacteria) I-5 ?
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Bacillus sp. (in: Bacteria) maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O
-
Bacillus sp. (in: Bacteria) I-5 maltodextrin
-
?
3.2.1.54 pullulan + H2O
-
Bacillus sp. (in: Bacteria) glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
-
?
3.2.1.54 pullulan + H2O
-
Bacillus sp. (in: Bacteria) I-5 glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
-
?
3.2.1.54 starch + H2O
-
Bacillus sp. (in: Bacteria) glucose + maltose
-
?
3.2.1.54 starch + H2O
-
Bacillus sp. (in: Bacteria) I-5 glucose + maltose
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 40 50 in the presence of Ca2+ Bacillus sp. (in: Bacteria)
3.2.1.54 50
-
pullulan, optimum temperature variable depending on the substrate Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 30 80
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 60
-
thermal activity more than 80% in presence of Ca2+ Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6 7 pullulan as substrate Bacillus sp. (in: Bacteria)
3.2.1.54 6
-
soluble starch, the optimum pH depending on the substrate Bacillus sp. (in: Bacteria)
3.2.1.54 7
-
beta-cyclodextrin as substrate Bacillus sp. (in: Bacteria)