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Literature summary extracted from

  • Bender, H.
    Purification and characterization of a cyclodextrin-degrading enzyme from Flavobacterium sp. (1993), Appl. Microbiol. Biotechnol., 39, 714-719.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 0.244
-
 beta-cyclodextrin
-
 Flavobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.54 cell bound
-
 Flavobacterium sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 62000
-
-
 Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Flavobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 purified to 94% homogeneity Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 60
-
 CM-Servacel, pH 6.7 Flavobacterium sp.
3.2.1.54 100
-
 beta-cyclodextrin as substrate Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
 Flavobacterium sp. glucose + maltose + maltotriose
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Flavobacterium sp. maltodextrin
-
 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
 Flavobacterium sp. glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose
-
 ?
3.2.1.54 maltodextrins + H2O 6 to 8 membered maltooligosaccharides Flavobacterium sp. maltose + glucose
-
 ?
3.2.1.54 maltoheptaose + H2O
-
 Flavobacterium sp. D-glucose + maltose + ?
-
 ?
3.2.1.54 maltohexaose + H2O
-
 Flavobacterium sp. maltotetraose + maltose
-
 ?
3.2.1.54 maltotriose + H2O
-
 Flavobacterium sp. ?
-
 ?
3.2.1.54 pullulan + H2O
-
 Flavobacterium sp. glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
-
 ?
3.2.1.54 starch + H2O
-
 Flavobacterium sp. glucose + maltose glucose minor early product, + maltotriose + maltotetraose ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 60
-
 enzyme is not thermostable, above 60°C more than 80% activity loss Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6 7.5
-
 Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 5.8 7.8
-
 Flavobacterium sp.