EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.41 | 2-mercaptoethanol | at 10 mM | Pyrococcus woesei | |
3.2.1.41 | dithiothreitol | at 10 mM | Pyrococcus woesei | |
3.2.1.41 | Triton X-100 | at 0.1% | Pyrococcus woesei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.41 | expression in Escherichia coli | Pyrococcus woesei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.41 | Cr3+ | - |
Pyrococcus woesei | |
3.2.1.41 | Cu2+ | - |
Pyrococcus woesei | |
3.2.1.41 | Cyclodextrins | - |
Pyrococcus woesei | |
3.2.1.41 | Fe2+ | - |
Pyrococcus woesei | |
3.2.1.41 | Guanidine HCl | at 0.25 M | Pyrococcus woesei | |
3.2.1.41 | iodoacetamide | at 10 mM | Pyrococcus woesei | |
3.2.1.41 | N-bromosuccinimide | - |
Pyrococcus woesei | |
3.2.1.41 | Ni2+ | - |
Pyrococcus woesei | |
3.2.1.41 | Sodium dodecyl sulfate | - |
Pyrococcus woesei | |
3.2.1.41 | Urea | at 6 M | Pyrococcus woesei | |
3.2.1.41 | Zn2+ | - |
Pyrococcus woesei |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.41 | Ca2+ | highly stimulated | Pyrococcus woesei | |
3.2.1.41 | Co2+ | - |
Pyrococcus woesei | |
3.2.1.41 | Mn2+ | slightly stimulated | Pyrococcus woesei | |
3.2.1.41 | Na2MoO4 | - |
Pyrococcus woesei | |
3.2.1.41 | Na2WO4 | - |
Pyrococcus woesei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 90000 | - |
gel electrophoresis | Pyrococcus woesei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.41 | amylopectin + H2O | Pyrococcus woesei | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
3.2.1.41 | pullulan + H2O | Pyrococcus woesei | - |
maltotriose + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.41 | Pyrococcus woesei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.41 | of the recombinant protein | Pyrococcus woesei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.41 | amylopectin + H2O | - |
Pyrococcus woesei | maltooligosaccharides + maltose | - |
? | |
3.2.1.41 | amylopectin + H2O | branched polysaccharides | Pyrococcus woesei | maltooligosaccharides + maltose | - |
? | |
3.2.1.41 | dextrin + H2O | - |
Pyrococcus woesei | maltooligosaccharides + maltose | - |
? | |
3.2.1.41 | pullulan + H2O | - |
Pyrococcus woesei | maltotriose + ? | - |
? | |
3.2.1.41 | soluble starch | - |
Pyrococcus woesei | maltotriose + maltose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.41 | amylase-pullulanase | - |
Pyrococcus woesei |
3.2.1.41 | pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Pyrococcus woesei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 100 | - |
- |
Pyrococcus woesei |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 80 | 120 | 40% activity at 120°C | Pyrococcus woesei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 100 | - |
after 4 h, 15% loss of activity | Pyrococcus woesei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 6 | - |
- |
Pyrococcus woesei |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 5.5 | 9 | - |
Pyrococcus woesei |