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Literature summary extracted from

  • Jang, I.S.; Kim, D.H.
    Purification and characterization of alpha-L-rhamnosidase from Bacteroides JY-6, a human intestinal bacterium (1996), Biol. Pharm. Bull., 19, 1546-1549.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 6-deoxy-D-glucose competitive Bacteroides JY-6
3.2.1.40 alpha-L-rhamnose competitive Bacteroides JY-6
3.2.1.40 Cu2+ 50% inhibition at 2 mM Bacteroides JY-6
3.2.1.40 Fe3+
-
 Bacteroides JY-6
3.2.1.40 L-fucose competitive Bacteroides JY-6
3.2.1.40 L-Mannose competitive Bacteroides JY-6
3.2.1.40 p-chlormercuriphenylsulfonic acid modification of cysteines Bacteroides JY-6
3.2.1.40 Pb2+
-
 Bacteroides JY-6
3.2.1.40 Zn2+
-
 Bacteroides JY-6

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.29
-
 p-nitrophenyl-alpha-L-rhamnopyranoside
-
 Bacteroides JY-6
3.2.1.40 0.82
-
 neohesperidin
-
 Bacteroides JY-6
3.2.1.40 0.89
-
 naringin
-
 Bacteroides JY-6
3.2.1.40 0.93
-
 poncirin
-
 Bacteroides JY-6
3.2.1.40 1.33
-
 hesperidin
-
 Bacteroides JY-6
3.2.1.40 1.44
-
 rutin
-
 Bacteroides JY-6
3.2.1.40 1.6
-
 saikosaponin C
-
 Bacteroides JY-6

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 120000
-
 2 * 120000, SDS-PAGE Bacteroides JY-6
3.2.1.40 240000
-
 gel filtration, SDS-PAGE Bacteroides JY-6

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 alpha-L-rhamnoside + H2O Bacteroides JY-6 alpha-L-rhamnose in glycolipids or glycosides alpha-L-rhamnose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Bacteroides JY-6
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 89.9
-
-
 Bacteroides JY-6

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4',5,7-trihydroxyflavanone 7-rhamnoglucoside + H2O
-
 Bacteroides JY-6 4',5,7-trihydroxyflavanone 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 alpha-L-rhamnoside + H2O alpha-L-rhamnose in glycolipids or glycosides Bacteroides JY-6 alpha-L-rhamnose + ?
-
 ?
3.2.1.40 hesperidin + H2O
-
 Bacteroides JY-6 hesperetin 7-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 naringin + H2O
-
 Bacteroides JY-6 beta-D-glucopyranosyl-2,3-dihydro-4',5,7-trihydroxyflavone + alpha-L-rhamnose
-
 ?
3.2.1.40 neohesperidin + H2O
-
 Bacteroides JY-6 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl alpha-L-rhamnoside + H2O
-
 Bacteroides JY-6 p-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O
-
 Bacteroides JY-6 p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 poncirin + H2O
-
 Bacteroides JY-6 poncirein + alpha-L-rhamnose
-
 ?
3.2.1.40 rutin + H2O
-
 Bacteroides JY-6 3-glycosylquercetin + alpha-L-rhamnose
-
 ?
3.2.1.40 saikosaponin C + H2O
-
 Bacteroides JY-6 ? + alpha-L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 dimer 2 * 120000, SDS-PAGE Bacteroides JY-6

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 7
-
-
 Bacteroides JY-6

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 5 9
-
 Bacteroides JY-6

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.40 2
-
 Cu2+
-
 Bacteroides JY-6