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Literature summary extracted from

  • Sanchez, M.; Nombela, C.; Villanueva, J.R.; Santos, T.
    Purification and partial characterization of a developmentally regulated 1,3-beta-glucanase from Penicillium italicum (1982), J. Gen. Microbiol., 128, 2047-2053.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 GSSG slight inhibition at 1 mM Penicillium italicum
3.2.1.39 Hg2+ marked inhibition at 1 mM Penicillium italicum
3.2.1.39 iodoacetamide slight inhibition Penicillium italicum
3.2.1.39 N-ethylmaleimide slight inhibition at 1 mM Penicillium italicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin value in mg/ml Penicillium italicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 65000 68000 SDS-PAGE, gel filtration Penicillium italicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Penicillium italicum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.39 glycoprotein
-
 Penicillium italicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 3step chromatography, isoelectric focusing Penicillium italicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 cell culture from derepressed mycelia Penicillium italicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 10.5
-
-
 Penicillium italicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 beta-glucan + H2O low activity Penicillium italicum ?
-
 ?
3.2.1.39 laminarin + H2O end-products are laminaritriose, laminaribiose and glucose Penicillium italicum D-glucose + ?
-
 ?
3.2.1.39 pustulan + H2O beta-1,6-glucan, low activity Penicillium italicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 monomer 1 * 65000-68000, SDS-PAGE, gel filtration Penicillium italicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 1,3-beta-glucanase I
-
 Penicillium italicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 50
-
-
 Penicillium italicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60
-
 sharp reduction of activity Penicillium italicum
3.2.1.39 70
-
 complete inactivation Penicillium italicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6
-
-
 Penicillium italicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 5
-
 65-70% retaining activity Penicillium italicum
3.2.1.39 7
-
 65-70% retaining activity Penicillium italicum