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Literature summary extracted from

  • Keen, N.T.; Yoshikawa, M.
    beta-1,3-Endoglucanase from soybean releases elicitor-active carbohydrates from fungus cell walls (1983), Plant Physiol., 71, 460-465.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 33000
-
 pH 10.7 isoform, SDS-PAGE, gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 cell wall glucan + H2O Glycine max from Phytophthora megasperma, carbohydrate release to elicit glyceollin accumulation in cotyledons reducing sugar + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Glycine max
-
 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 2step chromatography, isoelectric focusing Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 2.6
-
 pH10.7-isoform Glycine max

Storage Stability

EC Number Storage Stability Organism
3.2.1.39 retains full activity following lyophilization for 3 months if stored at -20°C, in solution stable if stored at -20°C Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 cell wall glucan + H2O from Phytophthora megasperma, carbohydrate release to elicit glyceollin accumulation in cotyledons Glycine max reducing sugar + ?
-
 ?
3.2.1.39 laminarin + H2O lower activity than on mycolaminarin Glycine max D-glucose + ?
-
 ?
3.2.1.39 mycolaminarin + H2O highest activity Glycine max reducing sugar no glucose release, approximately four glycosidic linkages hydrolyzed per molecule mycolaminarin, predominant product are tetrasaccharides ?
3.2.1.39 O-carboxymethyl-pachyman + H2O
-
 Glycine max ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 beta-1,3-endoglucanase two isoforms, pH 8.7 and pH 10.5 glucanases Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 65
-
 inactivation above Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5.3
-
-
 Glycine max