EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.38 | beta-D-fructose | promotes beta-D-fucosidase activity, not beta-D-glucosidase activity | Bifidobacterium breve clb | |
3.2.1.38 | beta-D-glucose | promotes beta-D-fucosidase activity at 50 mM, not beta-D-glucosidase activity | Bifidobacterium breve clb | |
3.2.1.38 | beta-D-mannose | promotes beta-D-fucosidase activity, not beta-D-glucosidase activity | Bifidobacterium breve clb | |
3.2.1.38 | beta-D-xylose | promotes beta-D-fucosidase activity, not beta-D-glucosidase activity | Bifidobacterium breve clb |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.38 | synthesis | synthesis of fucosylsugars using the transglycosylation activity | Bifidobacterium breve clb |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.38 | 1-amino-1-deoxy-D-glucose | - |
Bifidobacterium breve clb | |
3.2.1.38 | 1-butanol | inhibition above 50 mM | Bifidobacterium breve clb | |
3.2.1.38 | 1-propanol | inhibition above 50 mM | Bifidobacterium breve clb | |
3.2.1.38 | 4-O-beta-D-galactopyranosyl-D-glucose | - |
Bifidobacterium breve clb | |
3.2.1.38 | 4-O-beta-D-glucopyranosyl-D-glucose | cellobiose | Bifidobacterium breve clb | |
3.2.1.38 | acetone | inhibition at low concentrations | Bifidobacterium breve clb | |
3.2.1.38 | acetonitrile | inhibition at low concentrations | Bifidobacterium breve clb | |
3.2.1.38 | Ag+ | inhibition at 1 mM | Bifidobacterium breve clb | |
3.2.1.38 | beta-D-fructofuranosyl-alpha-D-glucopyranoside | - |
Bifidobacterium breve clb | |
3.2.1.38 | beta-D-fucose | inhibition of beta-D-glucosidase activity, beta-D-fucosidase activity hardly affected | Bifidobacterium breve clb | |
3.2.1.38 | beta-D-glucose | inhibition of beta-D-glucosidase activity, not beta-D-fucosidase activity | Bifidobacterium breve clb | |
3.2.1.38 | Cu2+ | inhibition at 1 mM | Bifidobacterium breve clb | |
3.2.1.38 | ethanol | inhibition above 50 mM | Bifidobacterium breve clb | |
3.2.1.38 | Hg2+ | inhibition at 1 mM | Bifidobacterium breve clb | |
3.2.1.38 | methanol | inhibition above 50 mM | Bifidobacterium breve clb | |
3.2.1.38 | p-chloromercuribenzoate | inhibition at 0.01 mM | Bifidobacterium breve clb |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.7 | - |
4-nitrophenyl-beta-D-fucoside | - |
Bifidobacterium breve | |
3.2.1.21 | 1.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Bifidobacterium breve | |
3.2.1.38 | 0.7 | - |
p-nitrophenyl-beta-D-fucoside | - |
Bifidobacterium breve clb | |
3.2.1.38 | 1.3 | - |
p-nitrophenyl-beta-D-glucoside | - |
Bifidobacterium breve clb |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.21 | extracellular | increasing activity by acclimation with cellobiose | Bifidobacterium breve | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 47000 | 48000 | native gradient PAGE | Bifidobacterium breve |
3.2.1.38 | 47000 | 48000 | native gradient PAGE, SDS-PAGE | Bifidobacterium breve clb |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.38 | 4-nitrophenyl beta-D-fucoside + H2O | Bifidobacterium breve clb | 80% activity compared to beta-D-glucoside | beta-D-fucose + 4-nitrophenol | - |
? | |
3.2.1.38 | 4-nitrophenyl beta-D-glucoside + H2O | Bifidobacterium breve clb | 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction | beta-D-glucose + 4-nitrophenol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Bifidobacterium breve | - |
- |
- |
3.2.1.38 | Bifidobacterium breve clb | - |
increased activity after acclimation with cellobiose | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | to homogeneity, chromatography steps | Bifidobacterium breve |
3.2.1.38 | to homogeneity by chromatography steps | Bifidobacterium breve clb |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | cell culture | - |
Bifidobacterium breve | - |
3.2.1.38 | cell culture | - |
Bifidobacterium breve clb | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.38 | 54.6 | - |
for beta-D-fucosidase activity | Bifidobacterium breve clb |
3.2.1.38 | 67.3 | - |
for beta-D-glucosidase activity | Bifidobacterium breve clb |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-fucoside + H2O | transferase activity for the beta-D-fucosyl group, not for a beta-D-glucosyl group, fucosidase activity specific for intestinal flora | Bifidobacterium breve | 4-nitrophenol + beta-D-fucose | - |
r | |
3.2.1.21 | 4-nitrophenyl beta-D-galactoside + H2O | low activity | Bifidobacterium breve | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Bifidobacterium breve | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Bifidobacterium breve | 4-nitrophenol + beta-D-xylose | - |
? | |
3.2.1.38 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | - |
Bifidobacterium breve clb | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
3.2.1.38 | 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | sophorose | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
3.2.1.38 | 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
3.2.1.38 | 4-methylumbelliferyl-beta-D-glucoside + H2O | increased activity after acclimation of bacteria to cellobiose medium | Bifidobacterium breve clb | 4-methylumbelliferone + beta-D-glucose | - |
? | |
3.2.1.38 | 4-nitrophenyl beta-D-fucoside + H2O | 80% activity compared to beta-D-glucoside | Bifidobacterium breve clb | beta-D-fucose + 4-nitrophenol | - |
? | |
3.2.1.38 | 4-nitrophenyl beta-D-glucoside + H2O | 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction | Bifidobacterium breve clb | beta-D-glucose + 4-nitrophenol | - |
? | |
3.2.1.38 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose, weak activity after prolonged incubation | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
3.2.1.38 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Bifidobacterium breve clb | beta-D-glucose + D-glucose | - |
? | |
3.2.1.38 | p-nitrophenyl-beta-D-fucoside + H2O | 80% activity compared to the activity towards beta-D-glucoside | Bifidobacterium breve clb | p-nitrophenol + beta-D-fucose | - |
? | |
3.2.1.38 | p-nitrophenyl-beta-D-fucoside + H2O | contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside | Bifidobacterium breve clb | p-nitrophenol + beta-D-fucose | - |
? | |
3.2.1.38 | p-nitrophenyl-beta-D-galactoside + H2O | low activity | Bifidobacterium breve clb | p-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.38 | p-nitrophenyl-beta-D-glucoside + H2O | - |
Bifidobacterium breve clb | p-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.38 | p-nitrophenyl-beta-D-xyloside + H2O | low activity | Bifidobacterium breve clb | p-nitrophenol + beta-D-xylose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | monomer | 1 * 47000-48000, SDS-PAGE, native gradient PAGE | Bifidobacterium breve |
3.2.1.38 | monomer | 1 * 47000-48000, SDS-PAGE, native PAGE | Bifidobacterium breve clb |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.38 | beta-D-glucosidase/beta-D-fucosidase | - |
Bifidobacterium breve clb |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 45 | - |
- |
Bifidobacterium breve |
3.2.1.38 | 45 | - |
- |
Bifidobacterium breve clb |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 40 | - |
stable up to | Bifidobacterium breve |
3.2.1.38 | 40 | - |
stable up to, 60% activity after incubation at 50°C | Bifidobacterium breve clb |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5.5 | - |
- |
Bifidobacterium breve |
3.2.1.38 | 5.5 | - |
- |
Bifidobacterium breve clb |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.38 | 5 | 7 | 90% activity between pH 5-7, 70% activity remained at pH 4.5 and pH 8.5 | Bifidobacterium breve clb |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5 | 7 | stable within, kept at 4°C | Bifidobacterium breve |