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Literature summary extracted from

  • Nunoura, N.; Ohdan, K.; Yano, T.; Yamamoto, K.; Kumagai, H.
    Purification and characterization of beta-D-glucosidase (beta-D-fucosidase) from Bifidobacterium berve clb acclimated to cellobiose (1996), Biosci. Biotechnol. Biochem., 60, 188-193.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.38 beta-D-fructose promotes beta-D-fucosidase activity, not beta-D-glucosidase activity Bifidobacterium breve clb
3.2.1.38 beta-D-glucose promotes beta-D-fucosidase activity at 50 mM, not beta-D-glucosidase activity Bifidobacterium breve clb
3.2.1.38 beta-D-mannose promotes beta-D-fucosidase activity, not beta-D-glucosidase activity Bifidobacterium breve clb
3.2.1.38 beta-D-xylose promotes beta-D-fucosidase activity, not beta-D-glucosidase activity Bifidobacterium breve clb

Application

EC Number Application Comment Organism
3.2.1.38 synthesis synthesis of fucosylsugars using the transglycosylation activity Bifidobacterium breve clb

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.38 1-amino-1-deoxy-D-glucose
-
Bifidobacterium breve clb
3.2.1.38 1-butanol inhibition above 50 mM Bifidobacterium breve clb
3.2.1.38 1-propanol inhibition above 50 mM Bifidobacterium breve clb
3.2.1.38 4-O-beta-D-galactopyranosyl-D-glucose
-
Bifidobacterium breve clb
3.2.1.38 4-O-beta-D-glucopyranosyl-D-glucose cellobiose Bifidobacterium breve clb
3.2.1.38 acetone inhibition at low concentrations Bifidobacterium breve clb
3.2.1.38 acetonitrile inhibition at low concentrations Bifidobacterium breve clb
3.2.1.38 Ag+ inhibition at 1 mM Bifidobacterium breve clb
3.2.1.38 beta-D-fructofuranosyl-alpha-D-glucopyranoside
-
Bifidobacterium breve clb
3.2.1.38 beta-D-fucose inhibition of beta-D-glucosidase activity, beta-D-fucosidase activity hardly affected Bifidobacterium breve clb
3.2.1.38 beta-D-glucose inhibition of beta-D-glucosidase activity, not beta-D-fucosidase activity Bifidobacterium breve clb
3.2.1.38 Cu2+ inhibition at 1 mM Bifidobacterium breve clb
3.2.1.38 ethanol inhibition above 50 mM Bifidobacterium breve clb
3.2.1.38 Hg2+ inhibition at 1 mM Bifidobacterium breve clb
3.2.1.38 methanol inhibition above 50 mM Bifidobacterium breve clb
3.2.1.38 p-chloromercuribenzoate inhibition at 0.01 mM Bifidobacterium breve clb

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.7
-
4-nitrophenyl-beta-D-fucoside
-
Bifidobacterium breve
3.2.1.21 1.3
-
4-nitrophenyl-beta-D-glucopyranoside
-
Bifidobacterium breve
3.2.1.38 0.7
-
p-nitrophenyl-beta-D-fucoside
-
Bifidobacterium breve clb
3.2.1.38 1.3
-
p-nitrophenyl-beta-D-glucoside
-
Bifidobacterium breve clb

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular increasing activity by acclimation with cellobiose Bifidobacterium breve
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 47000 48000 native gradient PAGE Bifidobacterium breve
3.2.1.38 47000 48000 native gradient PAGE, SDS-PAGE Bifidobacterium breve clb

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.38 4-nitrophenyl beta-D-fucoside + H2O Bifidobacterium breve clb 80% activity compared to beta-D-glucoside beta-D-fucose + 4-nitrophenol
-
?
3.2.1.38 4-nitrophenyl beta-D-glucoside + H2O Bifidobacterium breve clb 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction beta-D-glucose + 4-nitrophenol
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Bifidobacterium breve
-
-
-
3.2.1.38 Bifidobacterium breve clb
-
increased activity after acclimation with cellobiose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Bifidobacterium breve
3.2.1.38 to homogeneity by chromatography steps Bifidobacterium breve clb

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
Bifidobacterium breve
-
3.2.1.38 cell culture
-
Bifidobacterium breve clb
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.38 54.6
-
for beta-D-fucosidase activity Bifidobacterium breve clb
3.2.1.38 67.3
-
for beta-D-glucosidase activity Bifidobacterium breve clb

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O transferase activity for the beta-D-fucosyl group, not for a beta-D-glucosyl group, fucosidase activity specific for intestinal flora Bifidobacterium breve 4-nitrophenol + beta-D-fucose
-
r
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O low activity Bifidobacterium breve 4-nitrophenol + beta-D-galactose
-
?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Bifidobacterium breve 4-nitrophenol + D-glucopyranose
-
?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O low activity Bifidobacterium breve 4-nitrophenol + beta-D-xylose
-
?
3.2.1.38 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
-
Bifidobacterium breve clb 2-hydroxymethylphenol + beta-D-glucose
-
?
3.2.1.38 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
3.2.1.38 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
3.2.1.38 4-methylumbelliferyl-beta-D-glucoside + H2O increased activity after acclimation of bacteria to cellobiose medium Bifidobacterium breve clb 4-methylumbelliferone + beta-D-glucose
-
?
3.2.1.38 4-nitrophenyl beta-D-fucoside + H2O 80% activity compared to beta-D-glucoside Bifidobacterium breve clb beta-D-fucose + 4-nitrophenol
-
?
3.2.1.38 4-nitrophenyl beta-D-glucoside + H2O 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction Bifidobacterium breve clb beta-D-glucose + 4-nitrophenol
-
?
3.2.1.38 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose, weak activity after prolonged incubation Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
3.2.1.38 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O 80% activity compared to the activity towards beta-D-glucoside Bifidobacterium breve clb p-nitrophenol + beta-D-fucose
-
?
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside Bifidobacterium breve clb p-nitrophenol + beta-D-fucose
-
?
3.2.1.38 p-nitrophenyl-beta-D-galactoside + H2O low activity Bifidobacterium breve clb p-nitrophenol + beta-D-galactose
-
?
3.2.1.38 p-nitrophenyl-beta-D-glucoside + H2O
-
Bifidobacterium breve clb p-nitrophenol + beta-D-glucose
-
?
3.2.1.38 p-nitrophenyl-beta-D-xyloside + H2O low activity Bifidobacterium breve clb p-nitrophenol + beta-D-xylose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 47000-48000, SDS-PAGE, native gradient PAGE Bifidobacterium breve
3.2.1.38 monomer 1 * 47000-48000, SDS-PAGE, native PAGE Bifidobacterium breve clb

Synonyms

EC Number Synonyms Comment Organism
3.2.1.38 beta-D-glucosidase/beta-D-fucosidase
-
Bifidobacterium breve clb

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 45
-
-
Bifidobacterium breve
3.2.1.38 45
-
-
Bifidobacterium breve clb

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 40
-
stable up to Bifidobacterium breve
3.2.1.38 40
-
stable up to, 60% activity after incubation at 50°C Bifidobacterium breve clb

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
-
Bifidobacterium breve
3.2.1.38 5.5
-
-
Bifidobacterium breve clb

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.38 5 7 90% activity between pH 5-7, 70% activity remained at pH 4.5 and pH 8.5 Bifidobacterium breve clb

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5 7 stable within, kept at 4°C Bifidobacterium breve