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Literature summary extracted from

  • Zeng, Y.; Li, Y.; Gu, Y.; Zhang, S.
    Purification and characterization of a strictly specific beta-D-fucosidase from Aspergillus phoenicis (1992), Arch. Biochem. Biophys., 1, 226-230.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.38 2-mercaptoethanol activation Aspergillus phoenicis
3.2.1.38 beta-D-glucose strong activation Aspergillus phoenicis
3.2.1.38 glycerol activation Aspergillus phoenicis
3.2.1.38 Salicin activation Aspergillus phoenicis
3.2.1.38 Serum albumin activation Aspergillus phoenicis

General Stability

EC Number General Stability Organism
3.2.1.38 freezing and thawing inactivates Aspergillus phoenicis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.38 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
 Aspergillus phoenicis
3.2.1.38 4-O-beta-D-glucopyranosyl-D-glucose cellobiose Aspergillus phoenicis
3.2.1.38 Ag+ strong inhibition Aspergillus phoenicis
3.2.1.38 beta-D-fucose
-
 Aspergillus phoenicis
3.2.1.38 D-fucono-gamma-lactone
-
 Aspergillus phoenicis
3.2.1.38 dicarbonic acid diethyl ester
-
 Aspergillus phoenicis
3.2.1.38 Hg2+ strong inhibition Aspergillus phoenicis
3.2.1.38 iodoacetate strong inhibition Aspergillus phoenicis
3.2.1.38 N-bromosuccinimide
-
 Aspergillus phoenicis
3.2.1.38 N-ethylmaleimide strong inhibition Aspergillus phoenicis
3.2.1.38 p-chloromercuribenzoate strong inhibition Aspergillus phoenicis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.38 2.4
-
 p-nitrophenyl-beta-D-fucoside
-
 Aspergillus phoenicis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.38 57000
-
 SDS-PAGE, gel filtration Aspergillus phoenicis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.38 4-nitrophenyl beta-D-fucoside + H2O Aspergillus phoenicis strictly specific beta-D-fucose + 4-nitrophenol
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.38 Glycerol 5% v/v required during purification Aspergillus phoenicis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.38 Aspergillus phoenicis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.1.38 2-mercaptoethanol, 20 mM required during purification Aspergillus phoenicis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.38 to homogeneity by aqueous two phase separation, chromatography steps Aspergillus phoenicis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.38 cell culture
-
 Aspergillus phoenicis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.38 7.6
-
-
 Aspergillus phoenicis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.38 4-nitrophenyl beta-D-fucoside + H2O strictly specific Aspergillus phoenicis beta-D-fucose + 4-nitrophenol
-
 ?
3.2.1.38 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose, weak activity after prolonged incubation Aspergillus phoenicis beta-D-glucose + D-glucose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O high activity Aspergillus phoenicis p-nitrophenol + beta-D-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.38 monomer 1 * 57000, SDS-PAGE, gel filtration Aspergillus phoenicis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.38 40
-
-
 Aspergillus phoenicis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.38 30
-
 stable below Aspergillus phoenicis
3.2.1.38 45
-
 inactivation after 30 min Aspergillus phoenicis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.38 6
-
-
 Aspergillus phoenicis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.38 5.5 6.5
-
 Aspergillus phoenicis