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Literature summary extracted from

  • Kitpreechavanich, V.; Hayashi, M.; Nagai, S.
    Purification and characterization of extracellular beta-xylosidase and beta-glucosidase from Aspergillus fumigatus (1986), Agric. Biol. Chem., 50, 1703-1711.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+ slight inhibition at 2 mM Aspergillus fumigatus
3.2.1.21 Fe3+ 44% inhibition at 2 mM Aspergillus fumigatus
3.2.1.21 Hg2+ 70% inhibition at 2 mM Aspergillus fumigatus
3.2.1.21 p-chloromercuribenzoic acid 33.3% inhibition at 2 mM Aspergillus fumigatus
3.2.1.21 SDS strong inhibition at 2 mM Aspergillus fumigatus
3.2.1.37 Cu2+ strong inhibition at 2 mM Aspergillus fumigatus
3.2.1.37 Fe3+ slight inhibition at 2 mM Aspergillus fumigatus
3.2.1.37 Hg2+ complete inhibition at 2 mM Aspergillus fumigatus
3.2.1.37 p-chloromercuribenzoate complete inhibition at 2 mM Aspergillus fumigatus
3.2.1.37 SDS complete inhibition at 2 mM Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 1.4
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Aspergillus fumigatus
3.2.1.21 11.4
-
 gentiobiose
-
 Aspergillus fumigatus
3.2.1.21 24.8
-
 cellobiose
-
 Aspergillus fumigatus
3.2.1.37 2
-
 p-nitrophenyl beta-D-xyloside
-
 Aspergillus fumigatus
3.2.1.37 23.8
-
 xylobiose
-
 Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Aspergillus fumigatus
-
-
3.2.1.37 extracellular
-
 Aspergillus fumigatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 95000
-
 4 * 95000, SDS-PAGE Aspergillus fumigatus
3.2.1.21 380000
-
 gel filtration Aspergillus fumigatus
3.2.1.37 90000
-
 4 * 90000, SDS-PAGE Aspergillus fumigatus
3.2.1.37 360000
-
 gel filtration, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus fumigatus cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus fumigatus gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 xylobiose + H2O Aspergillus fumigatus
-
 D-xylose + beta-D-xylose
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O Aspergillus fumigatus
-
 beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O Aspergillus fumigatus
-
 beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Aspergillus fumigatus
-
-
-
3.2.1.37 Aspergillus fumigatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 to homogeneity, chromatography steps Aspergillus fumigatus
3.2.1.37 to homogeneity by 4step chromatography Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Aspergillus fumigatus
-
3.2.1.37 cell culture
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 99.6
-
-
 Aspergillus fumigatus
3.2.1.37 16.2
-
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Aspergillus fumigatus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Aspergillus fumigatus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O low activity Aspergillus fumigatus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus fumigatus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O low activity Aspergillus fumigatus 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus fumigatus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Aspergillus fumigatus beta-D-glucose + D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Aspergillus fumigatus 2 beta-D-glucose
-
 ?
3.2.1.21 methyl-beta-D-glucoside + H2O low activity Aspergillus fumigatus beta-D-glucose + methanol
-
 ?
3.2.1.21 xylobiose + H2O
-
 Aspergillus fumigatus D-xylose + beta-D-xylose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Aspergillus fumigatus p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O
-
 Aspergillus fumigatus beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Aspergillus fumigatus beta-D-xylose + D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 tetramer 4 * 95000, SDS-PAGE Aspergillus fumigatus
3.2.1.37 tetramer 4 * 90000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 65
-
-
 Aspergillus fumigatus
3.2.1.37 75
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 stable up to for 20 min Aspergillus fumigatus
3.2.1.37 65
-
 stable up to Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5
-
-
 Aspergillus fumigatus
3.2.1.37 4.5
-
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 2 8 stable within Aspergillus fumigatus
3.2.1.37 2 8 stable for 20 min at 50°C Aspergillus fumigatus