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Literature summary extracted from

  • Lachke, A.H.
    1,4-beta-D-Xylan xylohydrolase of Sclerotium rolfsii (1988), Methods Enzymol., 160, 679-684.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.37 no loss of activity if stored at -15°C at pH 4.5 Athelia rolfsii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ag+ 78% inhibition at 2 mM Athelia rolfsii
3.2.1.37 beta-D-xylose 8-15% inhibition at 1-3 mM Athelia rolfsii
3.2.1.37 Cu2+ 30% inhibition at 2 mM Athelia rolfsii
3.2.1.37 Hg2+ complete inhibition at 2 mM Athelia rolfsii
3.2.1.37 iodoacetamide highly inhibitory Athelia rolfsii
3.2.1.37 N-bromosuccinimide highly inhibitory at 1-2 mM Athelia rolfsii
3.2.1.37 p-hydroxymercuribenzoate highly inhibitory at 1-2 mM Athelia rolfsii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.038
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Athelia rolfsii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Athelia rolfsii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 170000 180000 gel filtration, SDS-PAGE Athelia rolfsii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylan + H2O Athelia rolfsii traces of xylanase activity beta-D-xylose + ?
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O Athelia rolfsii
-
 beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O Athelia rolfsii transxylosylation activity beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Athelia rolfsii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by 2step chromatography, isoelectric focusing Athelia rolfsii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 cell culture
-
 Athelia rolfsii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 1.25
-
-
 Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 o-nitrophenyl beta-D-xylopyranoside + H2O
-
 Athelia rolfsii o-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Athelia rolfsii p-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 xylan + H2O traces of xylanase activity Athelia rolfsii beta-D-xylose + ?
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O
-
 Athelia rolfsii beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O transxylosylation activity Athelia rolfsii beta-D-xylose + D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 monomer 1 * 170000-180000, gel filtration, SDS-PAGE Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 50
-
-
 Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 50
-
 retains 92% of its activity for 90 min Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4.5
-
-
 Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 4 5 stable within, not active below pH 2.5 or above pH 6.5 Athelia rolfsii