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Literature summary extracted from

  • Matsuo, M.; Yasui, T.
    beta-Xylosidases of several fungi (1988), Methods Enzymol., 160, 684-694.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.37 addition of Ca2+ protects against thermal inactivation Malbranchea pulchella var. sulfurea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Cu2+ strong inhibition Malbranchea pulchella var. sulfurea
3.2.1.37 Hg2+ complete inhibition Aspergillus nidulans
3.2.1.37 Hg2+ strong inhibition Malbranchea pulchella var. sulfurea
3.2.1.37 Hg2+ strong inhibition Trichoderma viride
3.2.1.37 N-bromosuccinimide complete inhibition at 1 mM Aspergillus nidulans
3.2.1.37 N-bromosuccinimide strong inhibition Malbranchea pulchella var. sulfurea
3.2.1.37 N-bromosuccinimide strong inhibition Trichoderma viride
3.2.1.37 p-chloromercuribenzoate strong inhibition Aspergillus nidulans
3.2.1.37 p-chloromercuribenzoate strong inhibition Malbranchea pulchella var. sulfurea
3.2.1.37 SDS complete inhibition Aspergillus nidulans
3.2.1.37 SDS strong inhibition Malbranchea pulchella var. sulfurea
3.2.1.37 Zn2+ strong inhibition Malbranchea pulchella var. sulfurea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Aspergillus nidulans
-
-
3.2.1.37 extracellular
-
 Trichoderma viride
-
-
3.2.1.37 extracellular
-
 Malbranchea pulchella var. sulfurea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 26000
-
 gel filtration Malbranchea pulchella var. sulfurea
3.2.1.37 101000 102000 SDS-PAGE, gel filtration Trichoderma viride
3.2.1.37 116000
-
 2 * 116000, SDS-PAGE Aspergillus nidulans
3.2.1.37 240000
-
 gel filtration, SDS-PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylan + H2O Malbranchea pulchella var. sulfurea
-
 beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O Aspergillus nidulans
-
 beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylobiose + H2O Trichoderma viride
-
 beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylobiose + H2O Malbranchea pulchella var. sulfurea
-
 beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aspergillus nidulans
-
-
-
3.2.1.37 Malbranchea pulchella var. sulfurea
-
-
-
3.2.1.37 Trichoderma viride
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein 4% carbohydrate moiety Aspergillus nidulans
3.2.1.37 glycoprotein 4.5% carbohydrate moiety Trichoderma viride

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by chromatography and electrophoretic steps Malbranchea pulchella var. sulfurea
3.2.1.37 to homogeneity by chromatography and isoelectric focusing Trichoderma viride
3.2.1.37 to homogeneity by chromatography, electrofocusing Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 cell culture
-
 Aspergillus nidulans
-
3.2.1.37 cell culture
-
 Trichoderma viride
-
3.2.1.37 cell culture
-
 Malbranchea pulchella var. sulfurea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 10.5
-
-
 Malbranchea pulchella var. sulfurea
3.2.1.37 10.8
-
-
 Trichoderma viride
3.2.1.37 62.9
-
-
 Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 benzyl-beta-D-xyloside + H2O low activity Aspergillus nidulans phenol + beta-D-xylose
-
 ?
3.2.1.37 butyl-beta-D-xyloside low activity Aspergillus nidulans butanol + beta-D-xylose
-
 ?
3.2.1.37 butyl-beta-D-xyloside low activity Trichoderma viride butanol + beta-D-xylose
-
 ?
3.2.1.37 cyclohexyl-beta-D-xyloside + H2O low activity Aspergillus nidulans cyclohexanol + beta-D-xylose
-
 ?
3.2.1.37 cyclohexyl-beta-D-xyloside + H2O low activity Trichoderma viride cyclohexanol + beta-D-xylose
-
 ?
3.2.1.37 methyl-beta-D-xyloside + H2O very low activity Trichoderma viride beta-D-xylose + methanol
-
 ?
3.2.1.37 methyl-beta-D-xyloside + H2O very low activity Trichoderma viride ?
-
 ?
3.2.1.37 o-nitrophenyl-beta-D-xyloside + H2O
-
 Aspergillus nidulans o-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 o-nitrophenyl-beta-D-xyloside + H2O
-
 Trichoderma viride o-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Aspergillus nidulans p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Trichoderma viride p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Malbranchea pulchella var. sulfurea p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 p-nitrophenyl-beta-D-xyloside + H2O beta-xylosidase-1 Malbranchea pulchella var. sulfurea p-nitrophenol + alpha-D-xylose inversion of anomeric configuration ?
3.2.1.37 xylan + H2O
-
 Malbranchea pulchella var. sulfurea beta-D-xylose + ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Aspergillus nidulans beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylobiose + H2O
-
 Trichoderma viride beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylobiose + H2O
-
 Malbranchea pulchella var. sulfurea beta-D-xylose + D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 dimer 2 * 116000, SDS-PAGE Aspergillus nidulans
3.2.1.37 monomer 1 * 101000-102000, SDS-PAGE, gel filtration Trichoderma viride
3.2.1.37 monomer 1 * 26000, SDS-PAGE, gel filtration Malbranchea pulchella var. sulfurea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 50
-
-
 Malbranchea pulchella var. sulfurea
3.2.1.37 55
-
-
 Aspergillus nidulans
3.2.1.37 55
-
-
 Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 50
-
 stable up to Aspergillus nidulans
3.2.1.37 50
-
 50% activity retained after 15 min Malbranchea pulchella var. sulfurea
3.2.1.37 55
-
 stable up to Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 3.5
-
-
 Trichoderma viride
3.2.1.37 4.5 5
-
 Aspergillus nidulans
3.2.1.37 6.2 6.8
-
 Malbranchea pulchella var. sulfurea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 3 4 stable for 30 min at 50°C Trichoderma viride
3.2.1.37 4.5 5 stable for 30 min at 50°C Aspergillus nidulans
3.2.1.37 6.3 6.7 stable within Malbranchea pulchella var. sulfurea