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Literature summary extracted from

  • Mujer, C.V.; Miller, A.R.
    Purification and properties of beta-xylosidase isoenzymes from cucumber seeds (1991), Physiol. Plant., 82, 367-376.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.37 xylose protects against thermal inactivation Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ag+ competitve inhibition Cucumis sativus
3.2.1.37 beta-D-xylose 43% competitive inhibition at 20 mM for beta-xylosidase A Cucumis sativus
3.2.1.37 Hg2+ competitive inhibition Cucumis sativus
3.2.1.37 iodoacetate inhibition of beta-xylosidase A Cucumis sativus
3.2.1.37 p-chloromercuribenzoate inhibition of beta-xylosidase A Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 1.5
-
 p-nitrophenyl beta-D-xylopyranoside xylosidase A Cucumis sativus
3.2.1.37 5.4
-
 p-nitrophenyl beta-D-xylopyranoside xylosidase B Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 intracellular
-
 Cucumis sativus 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 68000
-
 both beta-xylosidase A and B, SDS-PAGE, gel filtration Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylo-oligosaccharides + H2O Cucumis sativus beta-xylosidase A beta-D-xylose + ?
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O Cucumis sativus derived from xylan beta-D-xylose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Cucumis sativus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein
-
 Cucumis sativus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 near homogeneity for beta-xylosidase A, partial for beta-xylosidase B Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 seed
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 21.3
-
 beta-xylosidase A Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cucumis sativus p-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O beta-xylosidase A Cucumis sativus beta-D-xylose + ?
-
 ?
3.2.1.37 xylo-oligosaccharides + H2O derived from xylan Cucumis sativus beta-D-xylose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-xylosidase A
-
 Cucumis sativus
3.2.1.37 beta-xylosidase B
-
 Cucumis sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 50
-
 xylosidase B Cucumis sativus
3.2.1.37 68
-
 xylosidase A Cucumis sativus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 75
-
 inactivated after 30 min Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4.5
-
 both xylosidase A and B Cucumis sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 4 8 fully active, beta-xylosidase A Cucumis sativus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 3
-
 18% of original activity remains Cucumis sativus