EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.3 | expression in Pichia pastoris, Saccharomyces cerevisiae or Aspergillus niger. In Pichia pastoris the C320A/E400C double mutant is produced at 75% of the wild-type glucoamylase, while the corresponding single mutants are produced at 1% and 20% of the wild-type level, respectively | Aspergillus awamori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | 0.25 | - |
maltotriose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
3.2.1.3 | 0.28 | - |
maltotriose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
3.2.1.3 | 0.29 | - |
maltotriose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori | |
3.2.1.3 | 1.21 | - |
maltose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
3.2.1.3 | 1.82 | - |
maltose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
3.2.1.3 | 2.97 | - |
maltose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 80370 | - |
MALDI-MS | Aspergillus awamori |
3.2.1.3 | 82330 | - |
recombinant enzyme expressed in Pichia pastoris, matrix-assisted laser desorption ionization mass spectrometry | Aspergillus awamori |
3.2.1.3 | 82840 | - |
recombinant enzyme expressed in Aspergillus niger, MALDI-MS | Aspergillus awamori |
3.2.1.3 | 83870 | - |
recombinant enzyme expressed in Saccharomyces cerevisiae, matrix-assisted laser desorption ionization mass spectrometry | Aspergillus awamori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Aspergillus awamori | - |
wild-type and mutant enzyme C320A/E400C | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.3 | wild-type enzyme | Aspergillus awamori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
3.2.1.3 | maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
3.2.1.3 | maltohexaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
3.2.1.3 | maltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
3.2.1.3 | maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? | |
3.2.1.3 | maltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
3.2.1.3 | maltotriose + H2O | - |
Aspergillus awamori | maltose + glucose | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | additional information | - |
additional information | turnover numbers for the recombinant enzymes with maltose through maltoheptaose as substrates | Aspergillus awamori | |
3.2.1.3 | 6.8 | - |
maltose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori | |
3.2.1.3 | 10.7 | - |
maltose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
3.2.1.3 | 11.3 | - |
maltose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
3.2.1.3 | 27.6 | - |
maltotriose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori | |
3.2.1.3 | 30.9 | - |
maltotriose | recombinant enzyme expressed in Saccharomyces cerevisiae | Aspergillus awamori | |
3.2.1.3 | 33.5 | - |
maltotriose | recombinant enzyme expressed in Aspergillus niger | Aspergillus awamori | |
3.2.1.3 | 44.7 | - |
maltotetraose | recombinant enzyme expressed in Pichia pastoris | Aspergillus awamori |