Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liebl, W.; Brem, D.; Gotschlich, A.
    Analysis of the gene for beta-fructosidase (invertase, inulinase) of the hyperthermophilic bacterium Thermotoga maritima, and characterization of the enzyme expressed in Escherichia coli (1998), Appl. Microbiol. Biotechnol., 50, 55-64.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 Cu2+ 1 mM Thermotoga maritima
3.2.1.26 FeCl2
-
 Thermotoga maritima
3.2.1.26 MoCl2 1 mM Thermotoga maritima
3.2.1.26 additional information high substrate concentrations and also high levels of the cleavage products glucose and fructose do not severly inhibit sucrose inversion Thermotoga maritima
3.2.1.26 Zn2+ 1 mM ZnCl2 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 15
-
 raffinose
-
 Thermotoga maritima
3.2.1.26 19
-
 inulin
-
 Thermotoga maritima
3.2.1.26 64
-
 sucrose
-
 Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Thermotoga maritima
-
 MSB8
-
3.2.1.26 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
 MSB8
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 2390
-
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 inulin + H2O
-
 Thermotoga maritima fructose + ?
-
 ?
3.2.1.26 inulin + H2O
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 fructose + ?
-
 ?
3.2.1.26 levan + H2O
-
 Thermotoga maritima ?
-
 ?
3.2.1.26 levan + H2O
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
 ?
3.2.1.26 raffinose + H2O
-
 Thermotoga maritima fructose + melibiose
-
 ?
3.2.1.26 raffinose + H2O
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Thermotoga maritima D-fructose + D-glucose
-
 ?
3.2.1.26 sucrose + H2O
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 D-fructose + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 90 95 10 min assay Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.26 60 98 60°C: about 35% of maximal activity, 98°C: about 20% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 60
-
 14 days, 59% loss of activity Thermotoga maritima
3.2.1.26 70
-
 14 days, 76% loss of activity Thermotoga maritima
3.2.1.26 80
-
 5 h, retains at least 85% of its initial activity Thermotoga maritima
3.2.1.26 85
-
 5 h, 15% loss of activity Thermotoga maritima
3.2.1.26 90
-
 5 h, substantial loss of activity Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 140
-
 raffinose
-
 Thermotoga maritima
3.2.1.26 590
-
 inulin
-
 Thermotoga maritima
3.2.1.26 2600
-
 sucrose
-
 Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 5.5
-
 recombinant enzyme Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 4.2 7.1 more than 50% of maximal activity between pH 4.2 and pH 7.1 Thermotoga maritima