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Literature summary extracted from

  • Pressey, R.; Avants, J.K.
    Invertases in oat seedlings (1980), Plant Physiol., 65, 135-140.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 Ag+ 0.02 mM, complete inhibition of invertase I, 44% inhibition of invertase II Avena sativa
3.2.1.26 aniline 10 mM, 45% inhibition of invertase I, no inhibition of invertase II Avena sativa
3.2.1.26 Hg2+ 0.002 mM, 55% inhibition of invertase I, complete inhibition of invertase II Avena sativa
3.2.1.26 I2 0.004 mM, complete inhibition of invertase II, 9% inhibition of invertase I Avena sativa
3.2.1.26 PCMB 0.002 mM, 21% inhibition of invertase I, 79% inhibition of invertase II Avena sativa
3.2.1.26 pyridoxal 1 mM, 65% inhibition of invertase I, 25% inhibition of invertase II Avena sativa
3.2.1.26 pyridoxamine 10 mM, 28% inhibition of invertase I, 65% inhibition of invertase II Avena sativa
3.2.1.26 pyridoxine 2.5 mM, 70% inhibition of invertase I, 20% inhibition of invertase II Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 2.4
-
 sucrose invertase I Avena sativa
3.2.1.26 2.9
-
 raffinose invertase I Avena sativa
3.2.1.26 6.7
-
 sucrose invertase II Avena sativa
3.2.1.26 14
-
 stachyose invertase I Avena sativa
3.2.1.26 17
-
 raffinose invertase II Avena sativa
3.2.1.26 25
-
 stachyose invertase II Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 59000
-
 invertase I, gel filtration Avena sativa
3.2.1.26 108000
-
 invertase II, gel filtration Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Avena sativa
-
 2 invertases: I and II
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 coleoptile high invertase activity in the apical segment of the coleoptile, the level of activity decreases sharply with distance from the apex Avena sativa
-
3.2.1.26 leaf low activity Avena sativa
-
3.2.1.26 root low activity Avena sativa
-
3.2.1.26 seedling highest activity at the apex of the coleoptile, much lower in the primary leaf, mesocotyl and root Avena sativa
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.26 refrigerated, stable for 4 weeks Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 raffinose + H2O
-
 Avena sativa fructose + melibiose
-
 ?
3.2.1.26 stachyose + H2O
-
 Avena sativa ?
-
 ?
3.2.1.26 sucrose + H2O
-
 Avena sativa D-fructose + D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 30
-
 at pH 5 or higher, stable for at least 30 min, invertase II Avena sativa
3.2.1.26 47
-
 pH 4.5, 5 min, 50% loss of activity Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4.3
-
 invertase I Avena sativa
3.2.1.26 5
-
 invertase II Avena sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 3 5 pH 3.0: about 50% of maximal activity, pH 5.0: about 80% of maximal activity, invertase II Avena sativa
3.2.1.26 4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 20% of maximal activity Avena sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 3
-
 0°C, 30 min, stable, invertase I Avena sativa
3.2.1.26 3.5
-
 low stability at or below, invertase II Avena sativa