Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wan, C.C.; Muldrey, J.E.; Li, S.C.; Li, Y.T.
    beta-Mannosidase from the mushroom Polyporus sulfureus (1976), J. Biol. Chem., 251, 4384-4388.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 Ag+ 2 mM, complete inhibition Polyporus sulfureus
3.2.1.25 Cu2+
-
 Polyporus sulfureus
3.2.1.25 D-mannose
-
 Polyporus sulfureus
3.2.1.25 Fe3+
-
 Polyporus sulfureus
3.2.1.25 Hg2+
-
 Polyporus sulfureus
3.2.1.25 PMSF
-
 Polyporus sulfureus
3.2.1.25 Zn2+
-
 Polyporus sulfureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 1.6
-
 p-nitrophenyl-beta-D-mannoside
-
 Polyporus sulfureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 64000
-
 gel filtration Polyporus sulfureus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Polyporus sulfureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Polyporus sulfureus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.25 fruit body
-
 Polyporus sulfureus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 4.2
-
-
 Polyporus sulfureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 beta1-4-mannobiose + H2O
-
 Polyporus sulfureus ?
-
 ?
3.2.1.25 Man-(beta1-4)GlcNAc + H2O
-
 Polyporus sulfureus mannose + GlcNAc
-
 ?
3.2.1.25 Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
-
 Polyporus sulfureus mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
-
 ?
3.2.1.25 mannotriose + H2O
-
 Polyporus sulfureus ?
-
 ?
3.2.1.25 additional information the enzyme liberates mannose from core glycopeptide isolated from Taka-amylase A and alpha1-acid glycoprotein Polyporus sulfureus ?
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O
-
 Polyporus sulfureus p-nitrophenol + alpha-D-mannose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 2
-
 hydrolysis of Manbeta1-4GlcNAcbeta1-4GlcNAcbeta-Asn Polyporus sulfureus
3.2.1.25 2.8
-
 hydrolysis of p-nitrophenyl-beta-D-mannoside or beta-1,3-mannobiose Polyporus sulfureus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.25 2.8 9 25°C, 1 h, stable Polyporus sulfureus