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Literature summary extracted from

  • McCleary, B.V.
    beta-D-Mannosidase (1988), Methods Enzymol., 160, 614-619.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.25 analysis the enzyme together with alpha-galactosidase and endo-beta-D-mannanase, can be used to characterize the structures of more complex oligosacharides Helix pomatia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 Ag+
-
 Helix pomatia
3.2.1.25 Hg2+
-
 Helix pomatia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 0.91
-
 4-methylumbelliferyl beta-D-mannopyranoside
-
 Helix pomatia
3.2.1.25 1.43
-
 p-nitrophenyl-beta-D-mannopyranoside
-
 Helix pomatia
3.2.1.25 2.33
-
 o-nitrophenyl-beta-D-mannopyranoside
-
 Helix pomatia
3.2.1.25 3.22
-
 Naphthyl-beta-D-mannopyranoside
-
 Helix pomatia
3.2.1.25 12.5
-
 beta-D-mannotetraitol
-
 Helix pomatia
3.2.1.25 12.5
-
 Beta-D-mannohexaitol
-
 Helix pomatia
3.2.1.25 12.5
-
 beta-D-mannopentaitol
-
 Helix pomatia
3.2.1.25 12.5
-
 beta-D-mannotriitol
-
 Helix pomatia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 94000
-
-
 Helix pomatia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Helix pomatia
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.25 glycoprotein contains 3.1% carbohydrate Helix pomatia

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Helix pomatia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 additional information
-
-
 Helix pomatia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 4-methylumbelliferyl beta-D-mannoside + H2O
-
 Helix pomatia 4-methylumbelliferone + beta-D-mannose
-
 ?
3.2.1.25 beta-D-mannohexaitol + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.25 beta-D-mannopentaitol + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.25 beta-D-mannotetraitol + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.25 beta-D-mannotriitol + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.25 beta-mannotetraitol + H2O
-
 Helix pomatia ?
-
 ?
3.2.1.25 naphthyl-beta-D-mannopyranoside + H2O
-
 Helix pomatia 2-naphthol + beta-D-mannose
-
 ?
3.2.1.25 o-nitrophenyl-beta-D-mannopyranoside + H2O
-
 Helix pomatia o-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O p-nitrophenyl-beta-D-mannopyranoside Helix pomatia p-nitrophenol + alpha-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 ? x * 94000, SDS-PAGE Helix pomatia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.25 45
-
 stable to extended incubation at pH 4.5 Helix pomatia
3.2.1.25 50
-
 rapid loss of activity above Helix pomatia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 4
-
-
 Helix pomatia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.25 4.5
-
 stable to extended incubation at 45°C Helix pomatia
3.2.1.25 7
-
 unstable above pH 7 Helix pomatia