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Literature summary extracted from

  • Ram, D.; Tulsiani, P.; Touster, O.
    alpha-D-Mannosidase from golgi membranes of rat liver (1978), Methods Enzymol., 50, 500-505.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.24 2-mercaptoethanol
-
 Rattus sp.
3.2.1.24 ATP 25% activation at 1 mM Rattus sp.
3.2.1.24 bovine serum albumin
-
 Rattus sp.
3.2.1.24 CMP 25% activation at 1 mM Rattus sp.
3.2.1.24 dithiothreitol
-
 Rattus sp.
3.2.1.24 L-fucose 20% activation at 250 mM Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 alpha-D-mannopyranose 30% inhibition at 250 mM Rattus sp.
3.2.1.24 Cu2+ potent inhibitor Rattus sp.
3.2.1.24 Fe2+ potent inhibitor Rattus sp.
3.2.1.24 p-chloromercuribenzenesulfonic acid strong inhibition at 1 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 30
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 Golgi apparatus
-
 Rattus sp. 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+
-
 Rattus sp.
3.2.1.24 Zn2+
-
 Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 295000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 glycopeptide + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannopyranosyl-oligosaccharides + H2O Rattus sp. from yeast mannan, a tetrasaccharide terminating in a mannopyranosyl residue linked to C-3 of its neighbour is not hydrolysed alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.24 glycoprotein
-
 Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 3.1
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 glycopeptide + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannopyranosyl-oligosaccharides + H2O from yeast mannan, a tetrasaccharide terminating in a mannopyranosyl residue linked to C-3 of its neighbour is not hydrolysed Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 5.5
-
-
 Rattus sp.