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Literature summary extracted from

  • Swaminathan, N.; Matta, K.L.; Donoso, L.A.; Bahl, O.P.
    Glycosidases of Aspergillus niger. III. Purification and characterization of 1,2-alpha-mannosidase (1972), J. Biol. Chem., 247, 1775-1779.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 D-mannono-1,5-lactone weak inhibition Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 2
-
 2-O-alpha-D-mannopyranosyl-D-mannopyranose
-
 Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger highly specific for alpha-1,2-mannosidic linkages 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 2-O-alpha-D-mannotriose + H2O Aspergillus niger highly specific for alpha-1,2-mannosidic linkages alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger very low activity 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus niger very low activity alpha-D-mannopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 commercial preparation Rhozyme HP-150, crude enzyme preparation Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 91
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O highly specific for alpha-1,2-mannosidic linkages Aspergillus niger alpha-D-mannopyranose + D-mannopyranose
-
 ?
3.2.1.24 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O highly specific for alpha-1,2-mannosidic linkages Aspergillus niger 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 2-O-alpha-D-mannotriose + H2O highly specific for alpha-1,2-mannosidic linkages Aspergillus niger alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O very low activity Aspergillus niger 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O very low activity Aspergillus niger alpha-D-mannopyranose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 4.8
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 5 8 stable within Aspergillus niger