Any feedback?
Literature summary extracted from
- alpha-D-Mannosidase. Preparation and properties of free and insolubilized enzyme (1976), Biochim. Biophys. Acta, 429, 884-894.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.24 | 25-30% of activity remains in 6 M urea | Canavalia ensiformis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | D-glucuronic acid | slight inhibition at 10 mM | Canavalia ensiformis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 44000 | - |
2 * 66000 + 2 * 44000, SDS-PAGE | Canavalia ensiformis |
| 3.2.1.24 | 66000 | - |
2 * 66000 + 2 * 44000, SDS-PAGE | Canavalia ensiformis |
| 3.2.1.24 | 200000 | - |
gel filtration, sucrose density gradient centrifugation | Canavalia ensiformis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | glycopeptide + H2O | Canavalia ensiformis | from ovalbumin | alpha-D-mannopyranose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Canavalia ensiformis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | glycoprotein | - |
Canavalia ensiformis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | to homogeneity, chromatography steps | Canavalia ensiformis |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | 66% remaining activity after urea is removed from a Sepharose-immobilized enzyme | Canavalia ensiformis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | seed | - |
Canavalia ensiformis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 20 | - |
- |
Canavalia ensiformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | glycopeptide + H2O | from ovalbumin | Canavalia ensiformis | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Canavalia ensiformis | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | tetramer | 2 * 66000 + 2 * 44000, SDS-PAGE | Canavalia ensiformis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 75 | - |
continual increase of activity up to | Canavalia ensiformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.5 | - |
- |
Canavalia ensiformis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
