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Literature summary extracted from

  • Tulsiani, D.R.P.; Opheim, D.J.; Touster, O.
    Purification and characterization of alpha-D-mannosidase from rat liver golgi membranes (1977), J. Biol. Chem., 252, 3227-3233.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.24 2-mercaptoethanol slight activation up to 2.5 mM Rattus sp.
3.2.1.24 ATP 25% activation at 1 mM Rattus sp.
3.2.1.24 bovine serum albumin 35% activation at 0.125 mg/ml Rattus sp.
3.2.1.24 CMP 25% activation at 1 mM Rattus sp.
3.2.1.24 dithiothreitol nearly 60% activation up to 2.5 mM Rattus sp.
3.2.1.24 L-fucose 20% activation at 250 mM Rattus sp.
3.2.1.114 2-mercaptoethanol activation Rattus norvegicus
3.2.1.114 ATP 1 mM, 27% activation Rattus norvegicus
3.2.1.114 bovine serum albumin 0.125 mg/l, 35% activation Rattus norvegicus
3.2.1.114 CMP 1 mM, 25% activation Rattus norvegicus
3.2.1.114 dithiothreitol activation Rattus norvegicus
3.2.1.114 fucose 250 mM, 15-20% activation Rattus norvegicus
3.2.1.114 additional information not activated by glucose, galactose, AMP, ADP, GMP, GDP, GTP, CDP, CTP, UMP, UDP, UTP, cAMP, cGMP Rattus norvegicus

General Stability

EC Number General Stability Organism
3.2.1.114 4-nitrophenyl alpha-D-mannoside stabilizes Rattus norvegicus
3.2.1.114 requires presence of detergent, 0.1% Triton X-100, to maintain enzyme in soluble form Rattus norvegicus
3.2.1.114 stable to repeated freeze-thawing cycles Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 alpha-D-mannopyranose 30% inhibition at 250 mM Rattus sp.
3.2.1.24 Cu2+ potent inhibitor Rattus sp.
3.2.1.24 Fe2+ potent inhibitor Rattus sp.
3.2.1.24 p-chloromercuribenzenesulfonic acid strong inhibition at 1 mM Rattus sp.
3.2.1.114 Cu2+ strong Rattus norvegicus
3.2.1.114 Fe2+ strong Rattus norvegicus
3.2.1.114 mannose 250 mM, 30% inhibition Rattus norvegicus
3.2.1.114 additional information not inhibited by chelating agents, 0.1 mM 1,10-phenanthroline, 1 mM 2,2'-dipyridyl, 250 mM glucose, 250 mM galactose; not inhibited by EDTA; not inhibited by Mn2+, Mg2+ Rattus norvegicus
3.2.1.114 p-chloromercuriphenylsulfonate 1 mM, 80% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 30
-
p-nitrophenyl-alpha-D-mannopyranoside
-
Rattus sp.
3.2.1.114 30
-
4-nitrophenyl alpha-D-mannoside
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 Golgi apparatus
-
Rattus sp. 5794
-
3.2.1.114 Golgi membrane
-
Rattus norvegicus 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+ 60% activation up to 2.5 mM Rattus sp.
3.2.1.24 Zn2+ 30% activation up to 2.5 mM Rattus sp.
3.2.1.114 Co2+ activation Rattus norvegicus
3.2.1.114 additional information not activated by Na+ Rattus norvegicus
3.2.1.114 additional information not activated by Mg2+, Mn2+ Rattus norvegicus
3.2.1.114 Zn2+ activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 75000
-
4 * 75000, denaturating electrophoresis Rattus sp.
3.2.1.24 295000
-
gel filtration Rattus sp.
3.2.1.114 110000
-
sucrose density gradient centrifugation Rattus norvegicus
3.2.1.114 295000
-
tetrameric form, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.114 additional information Rattus norvegicus function in Golgi membranes: presumably glycopolymer metabolism ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-
3.2.1.114 Rattus norvegicus
-
male Wistar rats
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.24 glycoprotein
-
Rattus sp.
3.2.1.114 glycoprotein presumably Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Rattus sp.
3.2.1.114 97fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver
-
Rattus sp.
-
3.2.1.114 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 3.1
-
-
Rattus sp.
3.2.1.114 3.1
-
pH 5.5, 37°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.2.1.114 5°C or frozen, 0.1 M potassium phosphate buffer, pH 7.2, Triton X-100, 30 days, 20% loss of activity Rattus norvegicus
3.2.1.114 frozen, at least 2 months, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
?
3.2.1.114 4-nitrophenyl alpha-D-mannoside + H2O
-
Rattus norvegicus 4-nitrophenol + alpha-D-mannose
-
?
3.2.1.114 additional information function in Golgi membranes: presumably glycopolymer metabolism Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 tetramer 4 * 75000, denaturating electrophoresis Rattus sp.
3.2.1.114 tetramer 4 * 75000-80000, enzyme exists also in a relatively stable 150 kDa dimeric form, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 37
-
assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.114 37
-
stable at pH 7-9, unstable at acidic pH-values, Zn2+ stabilizes Rattus norvegicus
3.2.1.114 40
-
and below, 15 min stable, rapid inactivation above, Zn2+ or 0.125 mg/l bovine serum albumin stabilizes Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 5.5
-
-
Rattus sp.
3.2.1.114 5.5
-
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.114 4.2 6 pH 4.2: about half-maximal activity, pH 6: about 80% of maximal activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.114 4 6 unstable at 37°C, no activity below pH 4 Rattus norvegicus
3.2.1.114 7 9 stable at 37°C Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.114 Rattus norvegicus pH 3 to 10 gradient, isoelectric focusing
-
5.8