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Literature summary extracted from

  • Kaya, T.; Aikawa, M.; Matsumoto, T.
    Purification and properties of alpha-mannosidase from baker's yeast (1977), J. Biochem., 82, 1443-1449.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.24 Mannan from yeast, 80% activation at 10 mg/ml Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 iodoacetic acid slight inhibition at 0.1 mM Saccharomyces cerevisiae
3.2.1.24 methyl-alpha-D-mannopyranoside 37% inhibition at 0.1 mM Saccharomyces cerevisiae
3.2.1.24 NaN3 slight inhibition at 0.1 mM Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.23
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Saccharomyces cerevisiae
3.2.1.24 0.34
-
 methyl-alpha-D-mannopyranoside
-
 Saccharomyces cerevisiae
3.2.1.24 0.42
-
 phenyl-alpha-D-mannopyranoside
-
 Saccharomyces cerevisiae
3.2.1.24 0.67
-
 Mannotetraose
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Ca2+ 28% stimulation at 0.1 mM Saccharomyces cerevisiae
3.2.1.24 Mg2+ 50% stimulation at 0.1 mM Saccharomyces cerevisiae
3.2.1.24 Zn2+ 90% stimulation at 0.1 mM Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 450000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 mannan + H2O Saccharomyces cerevisiae very low activity alpha-D-mannose + ?
-
 ?
3.2.1.24 mannan + H2O Saccharomyces cerevisiae from yeast alpha-D-mannose + ?
-
 ?
3.2.1.24 mannotetraose + H2O Saccharomyces cerevisiae
-
 alpha-D-mannopyranose + mannotriose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 partial Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 cell culture
-
 Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 0.13
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.2.1.24 5°C, 50% loss of activity after 3 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 mannan + H2O very low activity Saccharomyces cerevisiae alpha-D-mannose + ?
-
 ?
3.2.1.24 mannan + H2O from yeast Saccharomyces cerevisiae alpha-D-mannose + ?
-
 ?
3.2.1.24 mannotetraose + H2O
-
 Saccharomyces cerevisiae alpha-D-mannopyranose + mannotriose
-
 ?
3.2.1.24 methyl-alpha-D-mannopyranoside + H2O
-
 Saccharomyces cerevisiae alpha-D-mannopyranose + methanol
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Saccharomyces cerevisiae p-nitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.24 phenyl-alpha-D-mannopyranoside + H2O
-
 Saccharomyces cerevisiae alpha-D-mannopyranose + phenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.24 60
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 44
-
 stable for 5 min Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6.4 6.8
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 6
-
 unstable below Saccharomyces cerevisiae