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Literature summary extracted from

  • Curdel, A.; Petek, F.
    Purification and properties of alpha-D-mannosidase from germinated seeds of Medicago sativa (alfalfa) (1980), Biochem. J., 185, 455-462.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Ag+
-
 Medicago sativa
3.2.1.24 Cd2+
-
 Medicago sativa
3.2.1.24 Cu2+
-
 Medicago sativa
3.2.1.24 EDTA addition of Zn2+ restores inhibitory effect Medicago sativa
3.2.1.24 Fe2+
-
 Medicago sativa
3.2.1.24 Fe3+
-
 Medicago sativa
3.2.1.24 Hg2+
-
 Medicago sativa
3.2.1.24 Zn2+
-
 Medicago sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 2.3
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Medicago sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 45000
-
 1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE Medicago sativa
3.2.1.24 50000
-
 1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE Medicago sativa
3.2.1.24 60000
-
 1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE Medicago sativa
3.2.1.24 75000
-
 1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE Medicago sativa
3.2.1.24 230000
-
 gel filtration, SDS-PAGE Medicago sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Medicago sativa
-
 alfalfa
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps, preparative electrophoresis Medicago sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 seed
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 215
-
-
 Medicago sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Medicago sativa p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 tetramer 1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE Medicago sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 60
-
 thermal activation up to Medicago sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 4.1
-
-
 Medicago sativa