EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag+ | - |
Lupinus angustifolius | |
3.2.1.22 | D-galactose | - |
Lupinus angustifolius | |
3.2.1.22 | Hg2+ | - |
Lupinus angustifolius | |
3.2.1.24 | Ag+ | 97% inhibition at 10 mM | Lupinus angustifolius | |
3.2.1.24 | alpha-conglutin | 11% inhibition at saturation | Lupinus angustifolius | |
3.2.1.24 | alpha-D-mannopyranose | 29% inhibition at 10 mM | Lupinus angustifolius | |
3.2.1.24 | Cu2+ | 10% inhibition at 10 mM, alpha-mannosidase A | Lupinus angustifolius | |
3.2.1.24 | EDTA | addition of Zn2+ restores inhibitory effect | Lupinus angustifolius | |
3.2.1.24 | Hg2+ | 45% inhibition at 10 mM | Lupinus angustifolius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.28 | - |
p-nitrophenyl alpha-D-galactose | alpha-galactosidase I | Lupinus angustifolius | |
3.2.1.22 | 0.56 | - |
p-nitrophenyl alpha-D-galactose | alpha-galactosidase II | Lupinus angustifolius | |
3.2.1.24 | 1.92 | - |
p-nitrophenyl-alpha-D-mannopyranoside | alpha-mannosidase A | Lupinus angustifolius | |
3.2.1.24 | 2.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | alpha-mannosidase B | Lupinus angustifolius |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.24 | protein storage vacuole | - |
Lupinus angustifolius | 326 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.24 | Zn2+ | maximum activity at 1 mM | Lupinus angustifolius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 17000 | - |
alpha-galactosidase II, gel filtration | Lupinus angustifolius |
3.2.1.22 | 70800 | - |
alpha-galactosidase I, gel filtration | Lupinus angustifolius |
3.2.1.24 | 300000 | - |
gel filtration, alpha-mannosidases A and B | Lupinus angustifolius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.24 | alpha-conglutin + H2O | Lupinus angustifolius | lupin seed storage glycoprotein, not active to beta- or gamma-conglutin | alpha-D-mannopyranose + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Lupinus angustifolius | - |
- |
- |
3.2.1.24 | Lupinus angustifolius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | - |
Lupinus angustifolius |
3.2.1.24 | to homogeneity, chromatography steps | Lupinus angustifolius |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.24 | cotyledon | alpha-mannosidases A and B | Lupinus angustifolius | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 285 | - |
alpha-galactosidase II | Lupinus angustifolius |
3.2.1.22 | 1600 | - |
alpha-galactosidase I | Lupinus angustifolius |
3.2.1.24 | 1.93 | - |
alpha-mannosidase B | Lupinus angustifolius |
3.2.1.24 | 15.94 | - |
alpha-mannosidase A | Lupinus angustifolius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | p-nitrophenyl-alpha-D-galactoside + H2O | - |
Lupinus angustifolius | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.24 | alpha-conglutin + H2O | lupin seed storage glycoprotein, not active to beta- or gamma-conglutin | Lupinus angustifolius | alpha-D-mannopyranose + ? | - |
? | |
3.2.1.24 | methyl-alpha-D-mannnopyranose + H2O | - |
Lupinus angustifolius | alpha-D-mannopyranose + methanol | - |
? | |
3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Lupinus angustifolius | p-nitrophenol + alpha-D-mannopyranose | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5.5 | - |
alpha-galactosidase II | Lupinus angustifolius |
3.2.1.22 | 6.5 | - |
alpha-galactosidase I | Lupinus angustifolius |
3.2.1.24 | 4 | - |
alpha-mannosidase A | Lupinus angustifolius |
3.2.1.24 | 4.6 | - |
alpha-mannosidase B | Lupinus angustifolius |