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Literature summary extracted from

  • Waln, K.T.; Poulton, J.E.
    Partial purification and characterization of an alpha-D-mannosidase from mature seeds of Prunus serotina Ehrh. (1987), Plant Sci., 53, 1-10.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Ag+ 70% inhibition at 1 mM Prunus serotina
3.2.1.24 D-mannono-1,4-lactone 11% inhibition at 10 mM Prunus serotina
3.2.1.24 D-mannono-1,5-lactone 50% competitive inhibition at 0.073 mM Prunus serotina
3.2.1.24 swainsonine competitive inhibition, Ki: 0.00036 mM Prunus serotina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 2.2
-
 4-methylumbelliferyl-alpha-D-mannopyranoside
-
 Prunus serotina
3.2.1.24 2.8
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Prunus serotina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Zn2+ 10% stimulation at 10 mM Prunus serotina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 150000
-
 gel filtration Prunus serotina

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Prunus serotina
-
 black cherry
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.24 glycoprotein
-
 Prunus serotina

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 partial Prunus serotina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 fruit
-
 Prunus serotina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Prunus serotina 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O most rapidly hydrolysed Prunus serotina p-nitrophenol + alpha-D-mannopyranose
-
 ?