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Literature summary extracted from
- Purification and partial characterization of soluble alpha-mannosidase isoforms from sunflower (Helianthus annuus L.) hypocotyls (1989), Plant Sci., 62, 11-19.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Ag+ | slight inhibition at 1 mM for isoforms I, II | Helianthus annuus |  |
| 3.2.1.24 | Ca2+ | slight inhibition at 1 mM | Helianthus annuus | |
| 3.2.1.24 | Cu2+ | complete inhibition at 1 mM | Helianthus annuus | |
| 3.2.1.24 | EDTA | complete inhibition at 1 mM, addition of 1 mM Zn2+ fully restores activity for isoforms II, to 69% for isoforms I | Helianthus annuus | |
| 3.2.1.24 | Fe2+ | complete inhibition at 1 mM | Helianthus annuus | |
| 3.2.1.24 | Hg2+ | slight inhibition at 1 mM for isoforms I,II | Helianthus annuus | |
| 3.2.1.24 | Mg2+ | slight inhibition at 1 mM | Helianthus annuus | |
| 3.2.1.24 | Mn2+ | complete inhibition at 1 mM | Helianthus annuus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 2.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | isoforms II | Helianthus annuus | |
| 3.2.1.24 | 3.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | isoforms I | Helianthus annuus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 260000 | - |
gel filtration, isoforms I and II | Helianthus annuus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Helianthus annuus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | glycoprotein | - |
Helianthus annuus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | to homogeneity, chromatography steps, chromatofocusing | Helianthus annuus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | hypocotyl | three isoforms I, II and III | Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 129.2 | - |
isoforms III | Helianthus annuus |
| 3.2.1.24 | 182 | - |
isoforms II | Helianthus annuus |
| 3.2.1.24 | 199.9 | - |
isoforms I | Helianthus annuus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.24 | 4°C, 25 mM sodium phosphate, pH 7.0, 9 months | Helianthus annuus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Helianthus annuus | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 50 | - |
- |
Helianthus annuus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.4 | - |
- |
Helianthus annuus |
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Release 2023.1 (January 2023)
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